Synthetic long reads reconstruction using molecular barcoding

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F22%3A00129621" target="_blank" >RIV/00216224:14740/22:00129621 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Synthetic long reads reconstruction using molecular barcoding

Popis výsledku v původním jazyce

The project aims to implement our newly developed method for the unique molecular identifiers (UMIs) introduction into oligonucleotides. This method can be used for RNA/DNA next generation sequencing (NGS) libraries preparation. The structure of the generated sequencing libraries with the introduced UMIs allows for a downstream long-reads reconstruction and PCR error correction resulting in accurately quantified ‚phased‘ genomic sequences or full-length transcripts. The potential range of the technology applications is very broad, and includes various fields, such as medical genomics, pharmacogenomics, molecular diagnostics, cancer genomics, and others. In this project, we focused on applying the method for the analysis 16S RNA sequencing (metagenomics) and immunogenomics analysis (TCR and BCR repertoires).

Název v anglickém jazyce

Synthetic long reads reconstruction using molecular barcoding

Popis výsledku anglicky

The project aims to implement our newly developed method for the unique molecular identifiers (UMIs) introduction into oligonucleotides. This method can be used for RNA/DNA next generation sequencing (NGS) libraries preparation. The structure of the generated sequencing libraries with the introduced UMIs allows for a downstream long-reads reconstruction and PCR error correction resulting in accurately quantified ‚phased‘ genomic sequences or full-length transcripts. The potential range of the technology applications is very broad, and includes various fields, such as medical genomics, pharmacogenomics, molecular diagnostics, cancer genomics, and others. In this project, we focused on applying the method for the analysis 16S RNA sequencing (metagenomics) and immunogenomics analysis (TCR and BCR repertoires).

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/TP01010039" target="_blank" >TP01010039: Posílení systému komercializace výsledků VaV na Masarykově univerzitě</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů