Stanovení kyseliny askorbové v lidské plazmě pomocí vysoceúčinné kapalinové chromatografie s UV detekcí s ohledem na její stabilitu

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F05%3A00002828" target="_blank" >RIV/00216275:25310/05:00002828 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of ascorbic acid in human plasma by high-performance liquid chromatography with ultraviolet detection with a view to stability

Popis výsledku v původním jazyce

A method for the measurement of ascorbic acid (AA) using high-performance liquid chromatography with ultraviolet detection and possibilities of protein precipitation with regard to stability and recovery are described. The aim of this work was to find out the most suitable precipitant reagent for the determination of AA in consideration of stability and recovery. The effectiveness of various protein precipitants was tested as follows. To plasma and AA solution (50.0 uM), the following different precipitant reagents were added: metaphosphoric acid (5%), perchloric acid (1.0 M), trichloroacetic acid (10%), sulfosalicylic acid (10%), acetonitrile with hydrochloric acid, acetonitrile with acetic acid, methanol with hydrochloric acid, and ethanol with hydrochloric acid. The only two protein precipitants that led to satisfactory recoveries of AA were metaphosphoric and perchloric acid. Stability of AA samples for analysis was investigated over 10 hours. Ascorbic acid samples extracted by met

Název v anglickém jazyce

Determination of ascorbic acid in human plasma by high-performance liquid chromatography with ultraviolet detection with a view to stability

Popis výsledku anglicky

A method for the measurement of ascorbic acid (AA) using high-performance liquid chromatography with ultraviolet detection and possibilities of protein precipitation with regard to stability and recovery are described. The aim of this work was to find out the most suitable precipitant reagent for the determination of AA in consideration of stability and recovery. The effectiveness of various protein precipitants was tested as follows. To plasma and AA solution (50.0 uM), the following different precipitant reagents were added: metaphosphoric acid (5%), perchloric acid (1.0 M), trichloroacetic acid (10%), sulfosalicylic acid (10%), acetonitrile with hydrochloric acid, acetonitrile with acetic acid, methanol with hydrochloric acid, and ethanol with hydrochloric acid. The only two protein precipitants that led to satisfactory recoveries of AA were metaphosphoric and perchloric acid. Stability of AA samples for analysis was investigated over 10 hours. Ascorbic acid samples extracted by met

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Clin. Chim. Acta suppl.

ISBN

0009-8981

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

220

Název nakladatele

Elsevier

Místo vydání

UK

Místo konání akce

Glasgow, Skotsko

Datum konání akce

8. 5. 2005

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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