Microextraction by Packed Sorbent (MEPS) as a Suitable Selective Method for L-Ascorbic Acid Determination in Beverages

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F12%3A39895227" target="_blank" >RIV/00216275:25310/12:39895227 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0308814612010114" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0308814612010114</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.foodchem.2012.06.039" target="_blank" >10.1016/j.foodchem.2012.06.039</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Microextraction by Packed Sorbent (MEPS) as a Suitable Selective Method for L-Ascorbic Acid Determination in Beverages

Popis výsledku v původním jazyce

In this study the modern microextraction by packed sorbent (MEPS) method followed by HPLC-UV analysis was optimised and evaluated for determination of L-ascorbic acid (AA) content in beverages. In this case 300 ?L of sample was passed through silica BIN(barrel insert and needle assembly) and entrapped AA was eluted by 60 ?L of methanol-water (10%, v/v) solution. Recovery of the proposed method for three concentration levels ranged between 97.46% and 106.88% with relative standard deviation 8.7% (low conc.), 2.49% (medium conc.) and 1.98% (high). Obtained results of AA contents in real samples were in the ranges 56.5-195.4 ?g/mL and 130.4-1090.0 ?g/mL for ice-tea and fruit juice samples, respectively. Proposed method was compared with iodometric titration and DPPH methods, but microextraction method was found to be more selective for AA determination in tested samples.

Název v anglickém jazyce

Microextraction by Packed Sorbent (MEPS) as a Suitable Selective Method for L-Ascorbic Acid Determination in Beverages

Popis výsledku anglicky

In this study the modern microextraction by packed sorbent (MEPS) method followed by HPLC-UV analysis was optimised and evaluated for determination of L-ascorbic acid (AA) content in beverages. In this case 300 ?L of sample was passed through silica BIN(barrel insert and needle assembly) and entrapped AA was eluted by 60 ?L of methanol-water (10%, v/v) solution. Recovery of the proposed method for three concentration levels ranged between 97.46% and 106.88% with relative standard deviation 8.7% (low conc.), 2.49% (medium conc.) and 1.98% (high). Obtained results of AA contents in real samples were in the ranges 56.5-195.4 ?g/mL and 130.4-1090.0 ?g/mL for ice-tea and fruit juice samples, respectively. Proposed method was compared with iodometric titration and DPPH methods, but microextraction method was found to be more selective for AA determination in tested samples.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Chemistry

ISSN

0308-8146

e-ISSN

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Svazek periodika

135

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

"1613?1618"

Kód UT WoS článku

000310396700106

EID výsledku v databázi Scopus

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