Detekce fusárií metodou PCR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F05%3A00003162" target="_blank" >RIV/00216275:25310/05:00003162 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection of Fusarium using the PCR Method

Popis výsledku v původním jazyce

Two methods of isolation of DNA were evaluated for successful identification of Fusarium species. The PCR was performed using primer specifically targeted to Tri5 gene. Extraction technique of DNA by Cenis (1992) and PCR procedure according to Edwards etal. (2001) was compared with DNA isolation and amplification using Plant PCR Kit. Sensitivity of the latter method was determined using reference strain of Fusarium graminearum CCM F-683 and selectivity was examined using reference cultures of differentgenera of fungi naturally occurred in food and feedstuff. Morphological features are important for species classification whereby PCR can distinguish between toxigenic and non-toxigenic fungi of Fusarium. These fungi could be detected within 2 days, while their identification by macroscopic and microscopic features takes 2-3 weeks. Thus the PCR technique has proved itself as reliable and rapid method for detection of toxigenic species of Fusarium.

Název v anglickém jazyce

Detection of Fusarium using the PCR Method

Popis výsledku anglicky

Two methods of isolation of DNA were evaluated for successful identification of Fusarium species. The PCR was performed using primer specifically targeted to Tri5 gene. Extraction technique of DNA by Cenis (1992) and PCR procedure according to Edwards etal. (2001) was compared with DNA isolation and amplification using Plant PCR Kit. Sensitivity of the latter method was determined using reference strain of Fusarium graminearum CCM F-683 and selectivity was examined using reference cultures of differentgenera of fungi naturally occurred in food and feedstuff. Morphological features are important for species classification whereby PCR can distinguish between toxigenic and non-toxigenic fungi of Fusarium. These fungi could be detected within 2 days, while their identification by macroscopic and microscopic features takes 2-3 weeks. Thus the PCR technique has proved itself as reliable and rapid method for detection of toxigenic species of Fusarium.

Druh

D - Stať ve sborníku

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F05%2F2106" target="_blank" >GA203/05/2106: Moderní instrumentace v chemické, potravinářské a klinické analýze</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Natural Sciences

ISBN

963 661 681 7

ISSN

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e-ISSN

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Počet stran výsledku

7

Strana od-do

417-423

Název nakladatele

University of Miskolc

Místo vydání

University of Miskolc

Místo konání akce

Miskolc Hungary

Datum konání akce

14. 8. 2005

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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