Toxinogenní fusária v potravinách a krmivech.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F06%3A00004294" target="_blank" >RIV/00216275:25310/06:00004294 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Toxigenic Fusarium in Food and Feed

Popis výsledku v původním jazyce

Contamination of small-grain cereals with the fungal species Fusarium graminearum, F. culmorum, F. poae and F. sporotrichioides is an important source of trichothecenes, zearalenone and other mycotoxins which cause serious diseases in human and animals.Early detection and control of these Fusarium species is crucial to prevent toxins entering the food chain and a useful tool in disease management practices. Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The extraction technique of DNA by Cenis (1992) and the PCR procedure according to Edwards et al. (2001)) were compared with the DNA isolation and amplification using the Plant PCR Kit. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff.

Název v anglickém jazyce

Toxigenic Fusarium in Food and Feed

Popis výsledku anglicky

Contamination of small-grain cereals with the fungal species Fusarium graminearum, F. culmorum, F. poae and F. sporotrichioides is an important source of trichothecenes, zearalenone and other mycotoxins which cause serious diseases in human and animals.Early detection and control of these Fusarium species is crucial to prevent toxins entering the food chain and a useful tool in disease management practices. Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The extraction technique of DNA by Cenis (1992) and the PCR procedure according to Edwards et al. (2001)) were compared with the DNA isolation and amplification using the Plant PCR Kit. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff.

Druh

D - Stať ve sborníku

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F05%2F2106" target="_blank" >GA203/05/2106: Moderní instrumentace v chemické, potravinářské a klinické analýze</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

20th International ICFMH Symposium Food Micro 2006

ISBN

961-90346-3-5

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

412

Název nakladatele

International Committee on Food Microbiology and Hygiene

Místo vydání

Bologna, Itálie

Místo konání akce

Bologna

Datum konání akce

29. 8. 2006

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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