Simultánní stanovení kyseliny vanilmandlové, homovanilové a 5-hydroxyindoloctové v moči pomocí HPLC s elektrochemickou detekcí

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F07%3A00006443" target="_blank" >RIV/00216275:25310/07:00006443 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Simultaneous determination of vanillylmandelic acid, homovanillic acid and 5-hydroxyindolacetic acid in urine by HPLC with electrochemical detection

Popis výsledku v původním jazyce

Background. Catecholamines are metabolized by monoamino oxidase and aldehyd dehydrogenase to vanillylmandelic acid (VMA) and homovanillic acid (HVA). The major metabolite of serotonin is 5-hydroxyindoleacetic acid (5-HIAA). A number of diseases are accompanied by increasing or decreased catecholamine metabolism. Methods. This method is based on selective isolation of VMA, HVA and 5-HIAA from urine samples on AG 1-X8 resin. Separation of VMA, HVA and 5-HIAA was achieved on reversed phase HPLC with isocratic elution and coulometric electrochemical detection (EG = 450 mV; E1 = 180 mV, gain range = 200 nA; E2 = 400 mV, gain range = 200 nA). Results. The analytical performance of this method is satisfactory with intra-assay CV 4.8% for VMA, CV 6.5% for HVAand 2.8% for 5-HIAA, inter-assay CV 8.2% for VMA, CV 10.2% for HVA and 8.4% for 5-HIAA, recovery 92.3% for VMA, 90.1% for HVA and 97.6% for 5-HIAA. The calibration curve was linear in the whole range tested (5.0 ? 500.0) umol/L. Conclusio

Název v anglickém jazyce

Simultaneous determination of vanillylmandelic acid, homovanillic acid and 5-hydroxyindolacetic acid in urine by HPLC with electrochemical detection

Popis výsledku anglicky

Background. Catecholamines are metabolized by monoamino oxidase and aldehyd dehydrogenase to vanillylmandelic acid (VMA) and homovanillic acid (HVA). The major metabolite of serotonin is 5-hydroxyindoleacetic acid (5-HIAA). A number of diseases are accompanied by increasing or decreased catecholamine metabolism. Methods. This method is based on selective isolation of VMA, HVA and 5-HIAA from urine samples on AG 1-X8 resin. Separation of VMA, HVA and 5-HIAA was achieved on reversed phase HPLC with isocratic elution and coulometric electrochemical detection (EG = 450 mV; E1 = 180 mV, gain range = 200 nA; E2 = 400 mV, gain range = 200 nA). Results. The analytical performance of this method is satisfactory with intra-assay CV 4.8% for VMA, CV 6.5% for HVAand 2.8% for 5-HIAA, inter-assay CV 8.2% for VMA, CV 10.2% for HVA and 8.4% for 5-HIAA, recovery 92.3% for VMA, 90.1% for HVA and 97.6% for 5-HIAA. The calibration curve was linear in the whole range tested (5.0 ? 500.0) umol/L. Conclusio

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Clinical Chemistry and Laboratory Medicine

ISBN

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ISSN

1434-6621

e-ISSN

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Počet stran výsledku

1

Strana od-do

"S212"

Název nakladatele

Walter de Gruyter GmbH & Co. KG

Místo vydání

Berlin

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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