Stanovení 2-oxokyselin odvozených od aminokyselin s rozvětveným řetězcem pomocí HPLC s fluorescenční detekcí

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F08%3A00007905" target="_blank" >RIV/00216275:25310/08:00007905 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of keto acids derived from branch chain amino acids using HPLC with fluorescence detection

Popis výsledku v původním jazyce

Background. The branched-chain keto acids (alpha-ketoisovaleric acid, KIV, alpha-ketoisocaproic acid, KIC and alpha-ketomethylvaleric acid, KMV) are the first degradation products of these amino acids produced by enzymatic transamination with branched-chain amino acids transaminase. Highly increased concentrations of branched-chain keto acids in human plasma and blood lead to the clinical syndrome of maple syrup urine disease (MSUD). Aim. The aim of this study was to develop rapid and simple HPLC methodfor measurement KIV, KIC, and KMV in plasma of blood donors and dried blood of newborns. Subject and methods. A HPLC method with fluorescence detection for the measurement of branched-chain keto acids in plasma and dried blood spots has been developed and evaluated. Samples of peripheral venous blood with EDTA as anticoagulant were obtained from a group of healthy blood donors (n = 70, 35 women in the age 27-41 years, mean age 37 years and 35 men in the age 28 43 years, mean age 38 year

Název v anglickém jazyce

Determination of keto acids derived from branch chain amino acids using HPLC with fluorescence detection

Popis výsledku anglicky

Background. The branched-chain keto acids (alpha-ketoisovaleric acid, KIV, alpha-ketoisocaproic acid, KIC and alpha-ketomethylvaleric acid, KMV) are the first degradation products of these amino acids produced by enzymatic transamination with branched-chain amino acids transaminase. Highly increased concentrations of branched-chain keto acids in human plasma and blood lead to the clinical syndrome of maple syrup urine disease (MSUD). Aim. The aim of this study was to develop rapid and simple HPLC methodfor measurement KIV, KIC, and KMV in plasma of blood donors and dried blood of newborns. Subject and methods. A HPLC method with fluorescence detection for the measurement of branched-chain keto acids in plasma and dried blood spots has been developed and evaluated. Samples of peripheral venous blood with EDTA as anticoagulant were obtained from a group of healthy blood donors (n = 70, 35 women in the age 27-41 years, mean age 37 years and 35 men in the age 28 43 years, mean age 38 year

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Program a sborník sympozia FONS 2008

ISBN

978-80-903879-2-8

ISSN

—

e-ISSN

—

Počet stran výsledku

2

Strana od-do

—

Název nakladatele

Stapro s.r.o.

Místo vydání

Pardubice

Místo konání akce

Pardubice

Datum konání akce

19. 9. 2008

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

—