Stanovení aminokyselin s rozvětveným řetězcem pomocí HPLC s fluorescenční detekcí

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F08%3A00007904" target="_blank" >RIV/00216275:25310/08:00007904 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of branched-chain amino acids by HPLC with fluorescence detection

Popis výsledku v původním jazyce

Background. The determination of branched-chain amino acids (valine, leucine and isoleucine), methionine, phenylalanine and tyrosine is presently the most reliable direct approach to the diagnosis of maple syrup urine disease (MSUD), hypermethioninemia,phenylketonuria (PKU) and tyrosinemia. Aim. The aim of this study was to develop rapid and simple HPLC method for measurement Val, Leu, Ile, Met, Phe and Tyr in plasma of blood donors and dried blood of newborns. Subject and methods. A HPLC method for measurement of branched-chain amino acids, Met, Phe and Tyr in plasma and whole blood has been developed and evaluated. Samples of peripheral venous blood with EDTA as anticoagulant were obtained from a group of healthy blood donors (n = 70, 35 women in the age 27-41 years, mean age 37 years and 35 men in the age 28 43 years, mean age 38 years). Plasma was separated from blood cells by centrifugation (1 700 x g, 15 min, 8 °C) and immediately stored at ?80 °C. Blood-spot samples from a grou

Název v anglickém jazyce

Determination of branched-chain amino acids by HPLC with fluorescence detection

Popis výsledku anglicky

Background. The determination of branched-chain amino acids (valine, leucine and isoleucine), methionine, phenylalanine and tyrosine is presently the most reliable direct approach to the diagnosis of maple syrup urine disease (MSUD), hypermethioninemia,phenylketonuria (PKU) and tyrosinemia. Aim. The aim of this study was to develop rapid and simple HPLC method for measurement Val, Leu, Ile, Met, Phe and Tyr in plasma of blood donors and dried blood of newborns. Subject and methods. A HPLC method for measurement of branched-chain amino acids, Met, Phe and Tyr in plasma and whole blood has been developed and evaluated. Samples of peripheral venous blood with EDTA as anticoagulant were obtained from a group of healthy blood donors (n = 70, 35 women in the age 27-41 years, mean age 37 years and 35 men in the age 28 43 years, mean age 38 years). Plasma was separated from blood cells by centrifugation (1 700 x g, 15 min, 8 °C) and immediately stored at ?80 °C. Blood-spot samples from a grou

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Program a sborník sympozia FONS 2008

ISBN

978-80-903879-2-8

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

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Název nakladatele

Stapro s.r.o.

Místo vydání

Pardubice

Místo konání akce

Pardubice

Datum konání akce

19. 9. 2008

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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