Determination of selected alpha-keto acids in dried blood samples using HPLC with fluorescence detection
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F22%3A39919066" target="_blank" >RIV/00216275:25310/22:39919066 - isvavai.cz</a>
Výsledek na webu
<a href="https://reader.elsevier.com/reader/sd/pii/S0731708522001595?token=3F716DF414A2A118CA22E35E713AE0D10EA5D0153B97DF6DA99A53B1C615E6B42DFAC79E26163B81A078B729588F7F49&originRegion=eu-west-1&originCreation=20221208125816" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0731708522001595?token=3F716DF414A2A118CA22E35E713AE0D10EA5D0153B97DF6DA99A53B1C615E6B42DFAC79E26163B81A078B729588F7F49&originRegion=eu-west-1&originCreation=20221208125816</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jpba.2022.114738" target="_blank" >10.1016/j.jpba.2022.114738</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Determination of selected alpha-keto acids in dried blood samples using HPLC with fluorescence detection
Popis výsledku v původním jazyce
The determination of alpha-keto acids derived from amino acids is currently the most reliable approach for the diagnosis of some congenital metabolic diseases. An HPLC method for the simultaneous measurement of selected alpha-keto acids in dried blood samples has been developed and evaluated. Blood spot samples from a group of healthy blood donors were collected onto #903 Specimen Collection Paper. Prior the separation, the alpha-keto acids were derivatized with 1,2-diamino-4,5-dimethoxybenzene to the corresponding 3-substituted-6,7-dimethoxy-2 (1 H)-quinoxalinol derivatives. For the separation, a reverse-phase column LichroCart 125-4, Purospher RP-18e, 5 mu m, was used. The mixture of 25% ACN in deionized water (mobile phase A) and 100% ACN (mobile phase B) were used for a gradient elution of alpha-keto acids derivatives. Analytical performance of this method is satisfactory for all alpha-keto acids. The intra-assay and inter-assay coefficients were below 10% and recoveries were close to 100%. We have developed relatively simple, rapid, selective and sufficiently sensitive HPLC method with fluorescence detection for the determination of selected alpha-keto acids in dried blood samples. The presented method is suitable for clinical testing purposes.
Název v anglickém jazyce
Determination of selected alpha-keto acids in dried blood samples using HPLC with fluorescence detection
Popis výsledku anglicky
The determination of alpha-keto acids derived from amino acids is currently the most reliable approach for the diagnosis of some congenital metabolic diseases. An HPLC method for the simultaneous measurement of selected alpha-keto acids in dried blood samples has been developed and evaluated. Blood spot samples from a group of healthy blood donors were collected onto #903 Specimen Collection Paper. Prior the separation, the alpha-keto acids were derivatized with 1,2-diamino-4,5-dimethoxybenzene to the corresponding 3-substituted-6,7-dimethoxy-2 (1 H)-quinoxalinol derivatives. For the separation, a reverse-phase column LichroCart 125-4, Purospher RP-18e, 5 mu m, was used. The mixture of 25% ACN in deionized water (mobile phase A) and 100% ACN (mobile phase B) were used for a gradient elution of alpha-keto acids derivatives. Analytical performance of this method is satisfactory for all alpha-keto acids. The intra-assay and inter-assay coefficients were below 10% and recoveries were close to 100%. We have developed relatively simple, rapid, selective and sufficiently sensitive HPLC method with fluorescence detection for the determination of selected alpha-keto acids in dried blood samples. The presented method is suitable for clinical testing purposes.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Pharmaceutical and Biomedical Analysis
ISSN
0731-7085
e-ISSN
1873-264X
Svazek periodika
214
Číslo periodika v rámci svazku
May
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
7
Strana od-do
114738
Kód UT WoS článku
000793640900014
EID výsledku v databázi Scopus
2-s2.0-85127593099