Determination of Total Glutathione in Dried Blood Spot Samples Using a High-Performance Liquid Chromatography
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900027" target="_blank" >RIV/00216275:25310/15:39900027 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1093/chromsci/bmu135" target="_blank" >http://dx.doi.org/10.1093/chromsci/bmu135</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/chromsci/bmu135" target="_blank" >10.1093/chromsci/bmu135</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Determination of Total Glutathione in Dried Blood Spot Samples Using a High-Performance Liquid Chromatography
Popis výsledku v původním jazyce
A method is described for the determination of total glutathione (TGSH) in dried blood spot (DBS) samples using high-performance liquid chromatography with fluorescence detection. Whole blood and DBS samples were obtained from a group of blood donors. After GSH reduction with dithiothreitol and protein precipitation with ethanol, the samples were derivatized with naphthalene-2,3-dicarboxaldehyde to form a very stable, highly fluorescent derivative. For the separation, a reversed phase HPLC method was used. The mixture of ethanol and deionized water (8 : 92, v/v) was used as a mobile phase. The analytical performance of this method was satisfactory: the intra-and interassay coefficients of variation were below 10%. Quantitative recoveries from spiked DBS samples were between 98.3 and 103.6%. The presented method is inexpensive and suitable for clinical testing purposes.
Název v anglickém jazyce
Determination of Total Glutathione in Dried Blood Spot Samples Using a High-Performance Liquid Chromatography
Popis výsledku anglicky
A method is described for the determination of total glutathione (TGSH) in dried blood spot (DBS) samples using high-performance liquid chromatography with fluorescence detection. Whole blood and DBS samples were obtained from a group of blood donors. After GSH reduction with dithiothreitol and protein precipitation with ethanol, the samples were derivatized with naphthalene-2,3-dicarboxaldehyde to form a very stable, highly fluorescent derivative. For the separation, a reversed phase HPLC method was used. The mixture of ethanol and deionized water (8 : 92, v/v) was used as a mobile phase. The analytical performance of this method was satisfactory: the intra-and interassay coefficients of variation were below 10%. Quantitative recoveries from spiked DBS samples were between 98.3 and 103.6%. The presented method is inexpensive and suitable for clinical testing purposes.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of chromatographic science
ISSN
0021-9665
e-ISSN
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Svazek periodika
53
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
7
Strana od-do
879-885
Kód UT WoS článku
000361837600007
EID výsledku v databázi Scopus
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