Determination of phenylalanine and tyrosine in plasma and dried blood samples using HPLC with fluorescence detection
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F09%3A00008563" target="_blank" >RIV/00216275:25310/09:00008563 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Determination of phenylalanine and tyrosine in plasma and dried blood samples using HPLC with fluorescence detection
Popis výsledku v původním jazyce
An HPLC method for the simultaneous measurement of phenylalanine and tyrosine in samples of dried blood spots and plasma has been developed and evaluated. We have used an inherent fluorescence of both phenylalanine and tyrosine. For the separation, a reverse-phase column LiChroCart 125-4, Purospher RP-18e, 5 um, was used. The mixture of ethanol and deionized water (5:95, v/v) was used as a mobile phase. Analytical performance of this method is satisfactory for both phenylalanine and tyrosine: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked plasma and blood samples were between 92.0 and 102.9%. The limit of detection was 10.0 and 5.0 umol/L, respectively. The preliminary reference ranges of phenylalanine and tyrosine in a group of newborns are 69.3 +/- 13.1 and 42.7 +/- 12.9 umol/L, in a group of blood donors are 68.4 +/- 9.9 and 52.1 +/- 10.9 umol/L.
Název v anglickém jazyce
Determination of phenylalanine and tyrosine in plasma and dried blood samples using HPLC with fluorescence detection
Popis výsledku anglicky
An HPLC method for the simultaneous measurement of phenylalanine and tyrosine in samples of dried blood spots and plasma has been developed and evaluated. We have used an inherent fluorescence of both phenylalanine and tyrosine. For the separation, a reverse-phase column LiChroCart 125-4, Purospher RP-18e, 5 um, was used. The mixture of ethanol and deionized water (5:95, v/v) was used as a mobile phase. Analytical performance of this method is satisfactory for both phenylalanine and tyrosine: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked plasma and blood samples were between 92.0 and 102.9%. The limit of detection was 10.0 and 5.0 umol/L, respectively. The preliminary reference ranges of phenylalanine and tyrosine in a group of newborns are 69.3 +/- 13.1 and 42.7 +/- 12.9 umol/L, in a group of blood donors are 68.4 +/- 9.9 and 52.1 +/- 10.9 umol/L.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2009
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Chromatography B
ISSN
1570-0232
e-ISSN
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Svazek periodika
877
Číslo periodika v rámci svazku
30
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
4
Strana od-do
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Kód UT WoS článku
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EID výsledku v databázi Scopus
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