Comparison of Various Second-Dimension Gradient Types in Comprehensive Two dimensional Liquid Chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F10%3A39882333" target="_blank" >RIV/00216275:25310/10:39882333 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of Various Second-Dimension Gradient Types in Comprehensive Two dimensional Liquid Chromatography

Popis výsledku v původním jazyce

Gradient elution provides significant improvement in peak capacity with respect to isocratic conditions. In the second dimension, gradients are limited to a short-time period available for separation. Various types of second-dimension gradients in comprehensive LCLC are compared: (i) ''full in fraction'', (ii) ''segment in fraction'' and (iii) ''continuously shifting'' gradients, applied in orthogonal LCLC separations of'phenolic acids and flavones on a polyethylene glycol column in the first dimensionand two types of porous shell fused-core C18 columns in the second dimension (Ascentis Express and Kinetex). The porous shell columns provide narrow bandwidths and fast second-dimension separations at moderate operating pressure that allows important savings of the overall separation time in comprehensive LCLC separations. The effects of the gradient type on the bandwidths, theoretical peak capacity, separation time and column pressure in the second dimension were investigated.

Název v anglickém jazyce

Comparison of Various Second-Dimension Gradient Types in Comprehensive Two dimensional Liquid Chromatography

Popis výsledku anglicky

Gradient elution provides significant improvement in peak capacity with respect to isocratic conditions. In the second dimension, gradients are limited to a short-time period available for separation. Various types of second-dimension gradients in comprehensive LCLC are compared: (i) ''full in fraction'', (ii) ''segment in fraction'' and (iii) ''continuously shifting'' gradients, applied in orthogonal LCLC separations of'phenolic acids and flavones on a polyethylene glycol column in the first dimensionand two types of porous shell fused-core C18 columns in the second dimension (Ascentis Express and Kinetex). The porous shell columns provide narrow bandwidths and fast second-dimension separations at moderate operating pressure that allows important savings of the overall separation time in comprehensive LCLC separations. The effects of the gradient type on the bandwidths, theoretical peak capacity, separation time and column pressure in the second dimension were investigated.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F07%2F0641" target="_blank" >GA203/07/0641: Nové analytické postupy v kombinovaných fázových systémech s vysokou separační kapacitou</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

33

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

16

Strana od-do

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Kód UT WoS článku

000278572000005

EID výsledku v databázi Scopus

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