Effects of the gradient profile, sample volume and solvent on the separation in very fast gradients, with special attention to the second-dimension gradient in comprehensive two-dimensional liquid chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892169" target="_blank" >RIV/00216275:25310/11:39892169 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chroma.2010.10.095" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2010.10.095</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2010.10.095" target="_blank" >10.1016/j.chroma.2010.10.095</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of the gradient profile, sample volume and solvent on the separation in very fast gradients, with special attention to the second-dimension gradient in comprehensive two-dimensional liquid chromatography

Popis výsledku v původním jazyce

Gradient elution improve peak capacity with respect to isocratic conditions and should be preferably used in 2D LC x LC in both dimensions. Gradient conditions spanning over a broad range are used with generic "full in fraction" (FIF) gradients. "Segmentin fraction" (SIF) gradients cover a limited gradient range. Optimization of the gradient profiles is important tool for achieving high 2D peak capacity and savings of the separation time. The transferred fraction volumes may significantly affect the bandwidths, especially at high elution strength of the fraction solvent, which may cause even band distortion or splitting in combined normal-phase (HILIC)-RP systems, but also in some 2D RP-RP systems. In the present work, the effects of the fast gradientprofile, of the sample volume and solvent on the elution time and bandwidths were investigated on a short column packed with fused-core porous-shell particles, providing narrow bandwidths and fast separations at moderate operating pressu

Název v anglickém jazyce

Effects of the gradient profile, sample volume and solvent on the separation in very fast gradients, with special attention to the second-dimension gradient in comprehensive two-dimensional liquid chromatography

Popis výsledku anglicky

Gradient elution improve peak capacity with respect to isocratic conditions and should be preferably used in 2D LC x LC in both dimensions. Gradient conditions spanning over a broad range are used with generic "full in fraction" (FIF) gradients. "Segmentin fraction" (SIF) gradients cover a limited gradient range. Optimization of the gradient profiles is important tool for achieving high 2D peak capacity and savings of the separation time. The transferred fraction volumes may significantly affect the bandwidths, especially at high elution strength of the fraction solvent, which may cause even band distortion or splitting in combined normal-phase (HILIC)-RP systems, but also in some 2D RP-RP systems. In the present work, the effects of the fast gradientprofile, of the sample volume and solvent on the elution time and bandwidths were investigated on a short column packed with fused-core porous-shell particles, providing narrow bandwidths and fast separations at moderate operating pressu

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

<a href="/cs/project/GA203%2F07%2F0641" target="_blank" >GA203/07/0641: Nové analytické postupy v kombinovaných fázových systémech s vysokou separační kapacitou</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1218

Číslo periodika v rámci svazku

15

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

1995-2006

Kód UT WoS článku

000289331200010

EID výsledku v databázi Scopus

—