Application of Electrochemical Sensors for Determination of Anticholinesterase Activity and Immobilization of Enzyme

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Application of Electrochemical Sensors for Determination of Anticholinesterase Activity and Immobilization of Enzyme

Popis výsledku v původním jazyce

Cholinesterases are important enzymes, which are responsible for the rapid elimination of acetyclholine after its releasing at cholinergic synapses. High activity of cholinesterases is present in patients with Alzheimer's disease. Therefore, testing of new inhibitors of cholinesterases are important. For determination of the inhibition efficiency several methods can be used. In our case, the anticholinesterase activity expressed by IC50 of five carbamate compounds were tested using screen-printed electrodes and square-wave voltammetry. The next part of our work was focused on testing of two types of immobilization of enzyme on the surface of screen-printed electrodes. Kinetic parameters (Km, Vm) were determined for immobilized and soluted enzyme. Obtained results were disscused.

Název v anglickém jazyce

Application of Electrochemical Sensors for Determination of Anticholinesterase Activity and Immobilization of Enzyme

Popis výsledku anglicky

Cholinesterases are important enzymes, which are responsible for the rapid elimination of acetyclholine after its releasing at cholinergic synapses. High activity of cholinesterases is present in patients with Alzheimer's disease. Therefore, testing of new inhibitors of cholinesterases are important. For determination of the inhibition efficiency several methods can be used. In our case, the anticholinesterase activity expressed by IC50 of five carbamate compounds were tested using screen-printed electrodes and square-wave voltammetry. The next part of our work was focused on testing of two types of immobilization of enzyme on the surface of screen-printed electrodes. Kinetic parameters (Km, Vm) were determined for immobilized and soluted enzyme. Obtained results were disscused.

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Sensing in Electroanalysis Vol. 7

ISBN

978-80-7395-563-2

ISSN

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e-ISSN

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Počet stran výsledku

24

Strana od-do

409-422

Název nakladatele

Univerzita Pardubice

Místo vydání

Pardubice

Místo konání akce

Pardubice

Datum konání akce

14. 11. 2012

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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