Cryptococcus laurentii Extracellular Biopolymer Production for Application in Wound Management

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39897838" target="_blank" >RIV/00216275:25310/14:39897838 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s12010-014-1105-x" target="_blank" >http://dx.doi.org/10.1007/s12010-014-1105-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12010-014-1105-x" target="_blank" >10.1007/s12010-014-1105-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cryptococcus laurentii Extracellular Biopolymer Production for Application in Wound Management

Popis výsledku v původním jazyce

Cryptococcus laurentii growth and extracellular polysaccharide (EPS) production in bioreactor were studied. Biomass yield 14.3 g/L and EPS synthesis 4.3 g/L in 144 h of submerged cultivation were achieved. EPS synthesis and cell growth had different optima. For EPS formation, pH 3, 25 A degrees C and low aeration (1 % { pO(2) { 10 %) were advantageous, while cell growth optimum was at pH 6, 20 A degrees C, and high aeration (pO(2) } 30 %). As medium pH changed from pH 3 to pH 6, glucuronic acid (GluAc)content in EPS increased, while galactose, xylose, and glucose decreased. Twenty-five degrees Celsius was optimal for GluAc containing polysaccharide synthesis, while lower temperature (15 A degrees C) increased glucose content in EPS. Aeration intensityand time of cultivation had little effect on EPS composition. Molecular mass distribution of raw C. laurentii EPS was determined by SEC-MALS as 1.352. The row EPS was composed of acidic glucuronoxylomannan for more than 85 %. In the in v

Název v anglickém jazyce

Cryptococcus laurentii Extracellular Biopolymer Production for Application in Wound Management

Popis výsledku anglicky

Cryptococcus laurentii growth and extracellular polysaccharide (EPS) production in bioreactor were studied. Biomass yield 14.3 g/L and EPS synthesis 4.3 g/L in 144 h of submerged cultivation were achieved. EPS synthesis and cell growth had different optima. For EPS formation, pH 3, 25 A degrees C and low aeration (1 % { pO(2) { 10 %) were advantageous, while cell growth optimum was at pH 6, 20 A degrees C, and high aeration (pO(2) } 30 %). As medium pH changed from pH 3 to pH 6, glucuronic acid (GluAc)content in EPS increased, while galactose, xylose, and glucose decreased. Twenty-five degrees Celsius was optimal for GluAc containing polysaccharide synthesis, while lower temperature (15 A degrees C) increased glucose content in EPS. Aeration intensityand time of cultivation had little effect on EPS composition. Molecular mass distribution of raw C. laurentii EPS was determined by SEC-MALS as 1.352. The row EPS was composed of acidic glucuronoxylomannan for more than 85 %. In the in v

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Biochemistry and Biotechnology

ISSN

0273-2289

e-ISSN

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Svazek periodika

174

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

1344-1353

Kód UT WoS článku

000342494300010

EID výsledku v databázi Scopus

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