Comprehensive two-dimensional liquid chromatography - mass spectrometry for lipidomic analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39899333" target="_blank" >RIV/00216275:25310/14:39899333 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comprehensive two-dimensional liquid chromatography - mass spectrometry for lipidomic analysis

Popis výsledku v původním jazyce

Lipids have numerous important functions in cells, tissues and body fluids, such as the formation of lipid bilayer, cell signaling, energy storage, etc. Lipidomics is a subset of metabolomics, which is aimed on the complex analysis of cellular lipid molecules isolated from cells, tissues or biological fluids and their biological roles with respect to health and disease states. Lipidomics contribute to the understanding how lipids work in a biological system (cellular physiology and pathology) [1]. One part of this work is focused on off-line two-dimensional liquid chromatography - mass spectrometry (2D-LC/MS) analysis of plasma samples, where the total lipid extract of plasma was separated into individual fractions of polar and nonpolar lipid classes.In the first dimension, 6 classes of polar lipids and 3 classes of nonpolar lipids were isolated by hydrophilic liquid chromatography (HILIC), where compounds are separated according to their polarity. Individual fractions were subsequent

Název v anglickém jazyce

Comprehensive two-dimensional liquid chromatography - mass spectrometry for lipidomic analysis

Popis výsledku anglicky

Lipids have numerous important functions in cells, tissues and body fluids, such as the formation of lipid bilayer, cell signaling, energy storage, etc. Lipidomics is a subset of metabolomics, which is aimed on the complex analysis of cellular lipid molecules isolated from cells, tissues or biological fluids and their biological roles with respect to health and disease states. Lipidomics contribute to the understanding how lipids work in a biological system (cellular physiology and pathology) [1]. One part of this work is focused on off-line two-dimensional liquid chromatography - mass spectrometry (2D-LC/MS) analysis of plasma samples, where the total lipid extract of plasma was separated into individual fractions of polar and nonpolar lipid classes.In the first dimension, 6 classes of polar lipids and 3 classes of nonpolar lipids were isolated by hydrophilic liquid chromatography (HILIC), where compounds are separated according to their polarity. Individual fractions were subsequent

Druh

O - Ostatní výsledky

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/LL1302" target="_blank" >LL1302: Hmotnostní spektrometrie při hledání lipidových biomarkerů pro včasnou diagnostiku rakoviny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů