Lipidomic analysis using hydrophilic interaction liquid chromatography microgradient fractionation of total lipid extracts

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F21%3A50018337" target="_blank" >RIV/62690094:18470/21:50018337 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60162694:G44__/21:00557168

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0021967321005045" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0021967321005045</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2021.462380" target="_blank" >10.1016/j.chroma.2021.462380</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Lipidomic analysis using hydrophilic interaction liquid chromatography microgradient fractionation of total lipid extracts

Popis výsledku v původním jazyce

Lipidomic samples are complex mixtures of structurally different species of a wide range of concen- trations providing challenges in their characterization. In this work, we present a proof of concept for the application of a simple microgradient liquid chromatography device on the detailed analysis of lipid classes. Our lipidomic analysis is based on a lipid class microgradient fractionation of a total lipid extract using an in-house-prepared hydrophilic interaction liquid chromatography microcolumn followed by RP- LC/MS of the collected lipid class fractions. The final fractionation method uses a 40-mm-long microcol- umn of 500 μm ID with silica stationary phase obtained from a commercially available chromatographic column and the microgradient of the mobile phase prepared in a microsyringe using methyl tert -butyl ether (MTBE) –methanol –water –ammonium acetate mixtures of various elution strengths. MTBE total lipid extract is directly separated by microgradient elution into lipid classes according to their polarity, which enables the collection of isolated fractions of most lipid classes. The method has been applied to the fractionation of porcine brain extract into nonpolar lipids, hexosylceramides, phosphoethanolamines, phosphocholines, sphingomyelins, and lysophosphocholines classes. Achieved repeatability, recovery, and advanced lipid coverage prove the applicability of the microgradient fractionation of total lipid extract for the comprehensive lipidomic analysis.

Název v anglickém jazyce

Lipidomic analysis using hydrophilic interaction liquid chromatography microgradient fractionation of total lipid extracts

Popis výsledku anglicky

Lipidomic samples are complex mixtures of structurally different species of a wide range of concen- trations providing challenges in their characterization. In this work, we present a proof of concept for the application of a simple microgradient liquid chromatography device on the detailed analysis of lipid classes. Our lipidomic analysis is based on a lipid class microgradient fractionation of a total lipid extract using an in-house-prepared hydrophilic interaction liquid chromatography microcolumn followed by RP- LC/MS of the collected lipid class fractions. The final fractionation method uses a 40-mm-long microcol- umn of 500 μm ID with silica stationary phase obtained from a commercially available chromatographic column and the microgradient of the mobile phase prepared in a microsyringe using methyl tert -butyl ether (MTBE) –methanol –water –ammonium acetate mixtures of various elution strengths. MTBE total lipid extract is directly separated by microgradient elution into lipid classes according to their polarity, which enables the collection of isolated fractions of most lipid classes. The method has been applied to the fractionation of porcine brain extract into nonpolar lipids, hexosylceramides, phosphoethanolamines, phosphocholines, sphingomyelins, and lysophosphocholines classes. Achieved repeatability, recovery, and advanced lipid coverage prove the applicability of the microgradient fractionation of total lipid extract for the comprehensive lipidomic analysis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA20-12289S" target="_blank" >GA20-12289S: Spojení superkritické fluidní chromatografie a hmotnostní spektrometrie jako nový nástroj pro charakterizaci lipidů a polárních metabolitů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF CHROMATOGRAPHY A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1653

Číslo periodika v rámci svazku

September

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

"Article Number: 462380"

Kód UT WoS článku

000685249900001

EID výsledku v databázi Scopus

2-s2.0-85111538995