Sensitive Voltammetric Sensor Based on Boron-Doped Diamond Electrode for Determination of the Chemotherapeutic Drug Methotrexate in Pharmaceutical and Biological Samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900150" target="_blank" >RIV/00216275:25310/15:39900150 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/elan.201400326" target="_blank" >http://dx.doi.org/10.1002/elan.201400326</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elan.201400326" target="_blank" >10.1002/elan.201400326</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sensitive Voltammetric Sensor Based on Boron-Doped Diamond Electrode for Determination of the Chemotherapeutic Drug Methotrexate in Pharmaceutical and Biological Samples

Popis výsledku v původním jazyce

A novel sensitive voltammetric method for the determination of an important chemotherapeutic drug, methotrexate, was designed using a bare boron-doped diamond electrode in connection with differential pulse voltammetry. Methotrexate provided one well developed oxidation peak suitable for analytical purposes at about + 1000 mV (vs. Ag/AgCl electrode) in an acidic medium. The voltammetric behavior of methotrexate in dependence of pH of the supporting electrolyte and scan rate has been investigated using cyclic voltammetry and DC voltammetry, respectively, to select the suitable experimental parameters. The optimum conditions for its determination using differential pulse voltammetry have been found to be 0.05 mol L-1 H2SO4 with a limit of detection of 1.0 x 10(-8) mol L-1 and a linear dynamic range from 5 x 10(-8) to 2 x 10(-5) mol L-1. Calculated values of relative standard deviations of repeated determinations (n }= 5) in various concentration levels of methotrexate did not exceed 4%.

Název v anglickém jazyce

Sensitive Voltammetric Sensor Based on Boron-Doped Diamond Electrode for Determination of the Chemotherapeutic Drug Methotrexate in Pharmaceutical and Biological Samples

Popis výsledku anglicky

A novel sensitive voltammetric method for the determination of an important chemotherapeutic drug, methotrexate, was designed using a bare boron-doped diamond electrode in connection with differential pulse voltammetry. Methotrexate provided one well developed oxidation peak suitable for analytical purposes at about + 1000 mV (vs. Ag/AgCl electrode) in an acidic medium. The voltammetric behavior of methotrexate in dependence of pH of the supporting electrolyte and scan rate has been investigated using cyclic voltammetry and DC voltammetry, respectively, to select the suitable experimental parameters. The optimum conditions for its determination using differential pulse voltammetry have been found to be 0.05 mol L-1 H2SO4 with a limit of detection of 1.0 x 10(-8) mol L-1 and a linear dynamic range from 5 x 10(-8) to 2 x 10(-5) mol L-1. Calculated values of relative standard deviations of repeated determinations (n }= 5) in various concentration levels of methotrexate did not exceed 4%.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/EE2.3.30.0021" target="_blank" >EE2.3.30.0021: Posílení excelentních týmů výzkumu a vývoje na Univerzitě Pardubice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electroanalysis

ISSN

1040-0397

e-ISSN

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Svazek periodika

27

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

42-51

Kód UT WoS článku

000347838300006

EID výsledku v databázi Scopus

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