Possibilities of retention prediction in fast gradient liquid chromatography. Part 3: Short silica monolithic columns

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900220" target="_blank" >RIV/00216275:25310/15:39900220 - isvavai.cz</a>

Výsledek na webu

<a href="http://apps.webofknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=2&SID=Z2XMp3XcgGvozkUKLG2&page=1&doc=1" target="_blank" >http://apps.webofknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=2&SID=Z2XMp3XcgGvozkUKLG2&page=1&doc=1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2015.07.070" target="_blank" >10.1016/j.chroma.2015.07.070</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Possibilities of retention prediction in fast gradient liquid chromatography. Part 3: Short silica monolithic columns

Popis výsledku v původním jazyce

We studied possibilities of prediction of the gradient elution data for alkylbenzenes, flavones and phenolic acids on two short octadecyl silica gel monolithic columns, namely a Chromolith Flash C18, 25 x 4.6 mm, and a "new generation" Chromolith High Resolution C18, 50 x 4.6 mm, in fast 1-2 min gradients. With fixed short gradient times and varying gradient ranges of acetonitrile concentration in water, high flow rates of the mobile phase (3-5 mL/min) could be used. The gradient elution data were predicted from four gradient models based on two-parameter and three-parameter isocratic retention equations. Various gradient retention models can be used for prediction of chromatograms and optimization of separation within a fixed gradient time. A two-parameter log log model introduced in 1974 and a three-parameter model introduced in 1980 provided slightly more accurate prediction than the Linear Solvent Strength (LSS) semi-logarithmic two-parameter model, most frequently used in reversed

Název v anglickém jazyce

Possibilities of retention prediction in fast gradient liquid chromatography. Part 3: Short silica monolithic columns

Popis výsledku anglicky

We studied possibilities of prediction of the gradient elution data for alkylbenzenes, flavones and phenolic acids on two short octadecyl silica gel monolithic columns, namely a Chromolith Flash C18, 25 x 4.6 mm, and a "new generation" Chromolith High Resolution C18, 50 x 4.6 mm, in fast 1-2 min gradients. With fixed short gradient times and varying gradient ranges of acetonitrile concentration in water, high flow rates of the mobile phase (3-5 mL/min) could be used. The gradient elution data were predicted from four gradient models based on two-parameter and three-parameter isocratic retention equations. Various gradient retention models can be used for prediction of chromatograms and optimization of separation within a fixed gradient time. A two-parameter log log model introduced in 1974 and a three-parameter model introduced in 1980 provided slightly more accurate prediction than the Linear Solvent Strength (LSS) semi-logarithmic two-parameter model, most frequently used in reversed

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Svazek periodika

1410

Číslo periodika v rámci svazku

July 2015

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

14

Strana od-do

76-89

Kód UT WoS článku

000360415900010

EID výsledku v databázi Scopus

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