Determination and Validation of the Antiviral Drugs Using Reverse Phase High Performance Liquid Chromatographic Method

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900645" target="_blank" >RIV/00216275:25310/15:39900645 - isvavai.cz</a>

Výsledek na webu

<a href="http://benthamscience.com/journals/current-drug-therapy/volume/9/issue/4/page/256/" target="_blank" >http://benthamscience.com/journals/current-drug-therapy/volume/9/issue/4/page/256/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2174/157488550904150525162237" target="_blank" >10.2174/157488550904150525162237</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination and Validation of the Antiviral Drugs Using Reverse Phase High Performance Liquid Chromatographic Method

Popis výsledku v původním jazyce

A new, simple, rapid, accurate and reproducible reverse phase high performance liquid chromatographic method with UV detection was developed and used for the estimation of abacavir sulphate and fosamprenavir in bulk and in their pharmaceutical dosage forms. High performance liquid chromatography was carried out on a Waters Spherisorb ODSI (250x4.6mm with 5 ?m particle size) column with a flow rate 1.0 mL.min-1 using a mobile phase consisting of methanol: phosphate buffer (80:20, v/v) containing 15mM phosphoric acid at pH 6.2. The detection was carried out at 275 nm and the run time was 5 min. For the sensitive determination of abacavir, fosamprenavir was used as an internal standard and for determination of fosamprenavir vice versa. Using these conditions, both antiviral drugs were analyzed simultaneously with the same method and same chromatographic conditions. The linearity was found to be in the range of 0.25-75 ?g.min-1 and 0.50-75 ?g.min-1 for abacavir and fosamprenavir, respectiv

Název v anglickém jazyce

Determination and Validation of the Antiviral Drugs Using Reverse Phase High Performance Liquid Chromatographic Method

Popis výsledku anglicky

A new, simple, rapid, accurate and reproducible reverse phase high performance liquid chromatographic method with UV detection was developed and used for the estimation of abacavir sulphate and fosamprenavir in bulk and in their pharmaceutical dosage forms. High performance liquid chromatography was carried out on a Waters Spherisorb ODSI (250x4.6mm with 5 ?m particle size) column with a flow rate 1.0 mL.min-1 using a mobile phase consisting of methanol: phosphate buffer (80:20, v/v) containing 15mM phosphoric acid at pH 6.2. The detection was carried out at 275 nm and the run time was 5 min. For the sensitive determination of abacavir, fosamprenavir was used as an internal standard and for determination of fosamprenavir vice versa. Using these conditions, both antiviral drugs were analyzed simultaneously with the same method and same chromatographic conditions. The linearity was found to be in the range of 0.25-75 ?g.min-1 and 0.50-75 ?g.min-1 for abacavir and fosamprenavir, respectiv

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Current Drug Therapy

ISSN

1574-8855

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

AE - Spojené arabské emiráty

Počet stran výsledku

5

Strana od-do

256-260

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-84930509693