Determination of selected amino acids in serum of patients with liver disease

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F16%3A39902087" target="_blank" >RIV/00216275:25310/16:39902087 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.17219/acem/63751" target="_blank" >http://dx.doi.org/10.17219/acem/63751</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17219/acem/63751" target="_blank" >10.17219/acem/63751</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of selected amino acids in serum of patients with liver disease

Popis výsledku v původním jazyce

Background. The determination of amino acids can be a reliable approach for extended diagnosis of liver diseases. This is because liver disease can be a cause of impaired amino acid metabolism. Therefore, a method for the determination of serum amino acids, applicable for clinical purposes, is necessary. Objectives. The aim of this study was to find differences in the levels of selected amino acids between patients with liver disease and a control group. Material and Methods. Samples of peripheral venous blood were obtained from a group of patients with liver disease (n = 131, 59 women at an average age of 60 years and 72 men at an average age of 52 years) and a control group (n = 105, 47 women at an average age of 62 years and 58 men at an average age of 58 years). Before the separation, the amino acids were derivatized with naphthalene-2,3-dicarboxaldehyde. For the separation, reverse phase column was used. The effluent was monitored with a fluorescence detector. Results. There were significant differences in the concentrations of some amino acids between the patients and the control group, but also between women and men. Correlations between some amino acids and markers of liver blood tests and lipid metabolism were observed. Conclusions. A simple, relatively rapid and selective HPLC method with fluorescence detection for the determination of selected amino acids in serum has been developed.

Název v anglickém jazyce

Determination of selected amino acids in serum of patients with liver disease

Popis výsledku anglicky

Background. The determination of amino acids can be a reliable approach for extended diagnosis of liver diseases. This is because liver disease can be a cause of impaired amino acid metabolism. Therefore, a method for the determination of serum amino acids, applicable for clinical purposes, is necessary. Objectives. The aim of this study was to find differences in the levels of selected amino acids between patients with liver disease and a control group. Material and Methods. Samples of peripheral venous blood were obtained from a group of patients with liver disease (n = 131, 59 women at an average age of 60 years and 72 men at an average age of 52 years) and a control group (n = 105, 47 women at an average age of 62 years and 58 men at an average age of 58 years). Before the separation, the amino acids were derivatized with naphthalene-2,3-dicarboxaldehyde. For the separation, reverse phase column was used. The effluent was monitored with a fluorescence detector. Results. There were significant differences in the concentrations of some amino acids between the patients and the control group, but also between women and men. Correlations between some amino acids and markers of liver blood tests and lipid metabolism were observed. Conclusions. A simple, relatively rapid and selective HPLC method with fluorescence detection for the determination of selected amino acids in serum has been developed.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Advances in Clinical and Experimental medicine

ISSN

1899-5276

e-ISSN

—

Svazek periodika

25

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

13

Strana od-do

1227-1239

Kód UT WoS článku

000392944200013

EID výsledku v databázi Scopus

2-s2.0-85006728978