Development of an online solid-phase extraction with liquid chromatography method based on polymer monoliths for the determination of dopamine
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F16%3A39915935" target="_blank" >RIV/00216275:25310/16:39915935 - isvavai.cz</a>
Výsledek na webu
<a href="http://apps.webofknowledge.com/Search.do?product=WOS&SID=E2PbjPruZIf4crFxEcX&search_mode=GeneralSearch&prID=0248833b-ce7c-42f9-83d0-a58fdb3d5672" target="_blank" >http://apps.webofknowledge.com/Search.do?product=WOS&SID=E2PbjPruZIf4crFxEcX&search_mode=GeneralSearch&prID=0248833b-ce7c-42f9-83d0-a58fdb3d5672</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201600818" target="_blank" >10.1002/jssc.201600818</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Development of an online solid-phase extraction with liquid chromatography method based on polymer monoliths for the determination of dopamine
Popis výsledku v původním jazyce
Porous polymer monoliths have been used to develop an online solid-phase extraction with liquid chromatography method for determination of dopamine in urine as well as for a continuous monitoring of dopamine in flowing system. A polymerization mixture containing 4-vinylphenylboronic acid monomer has been used to prepare a trapping column based on specific ring formation reaction with dopamine cis-diol functionality. Additionally, a monolithic stationary phase with zwitterion functionality has been used to prepare capillary column for the separation of dopamine. Experimental conditions including molarity, pH, and flow rate of the loading buffer together with a valve switching time have been optimized to provide the highest recovery for dopamine. Experimental setup has been used to determine dopamine in a urine. By using both calibration curve and standard addition method, the dopamine level was determined to be 1.19 and 1.28 mg/L, respectively. Further, we have used experimental design to optimize coupling of two extraction monolithic loops to separation capillary column with monolithic phase for a comprehensive monitoring of dopamine. After multivariate analysis, sample loading flow-rate and a flow-rate of flushing buffer were selected as the most significant variables. Optimized experimental setup was applied to continuously monitor dopamine degradation.
Název v anglickém jazyce
Development of an online solid-phase extraction with liquid chromatography method based on polymer monoliths for the determination of dopamine
Popis výsledku anglicky
Porous polymer monoliths have been used to develop an online solid-phase extraction with liquid chromatography method for determination of dopamine in urine as well as for a continuous monitoring of dopamine in flowing system. A polymerization mixture containing 4-vinylphenylboronic acid monomer has been used to prepare a trapping column based on specific ring formation reaction with dopamine cis-diol functionality. Additionally, a monolithic stationary phase with zwitterion functionality has been used to prepare capillary column for the separation of dopamine. Experimental conditions including molarity, pH, and flow rate of the loading buffer together with a valve switching time have been optimized to provide the highest recovery for dopamine. Experimental setup has been used to determine dopamine in a urine. By using both calibration curve and standard addition method, the dopamine level was determined to be 1.19 and 1.28 mg/L, respectively. Further, we have used experimental design to optimize coupling of two extraction monolithic loops to separation capillary column with monolithic phase for a comprehensive monitoring of dopamine. After multivariate analysis, sample loading flow-rate and a flow-rate of flushing buffer were selected as the most significant variables. Optimized experimental setup was applied to continuously monitor dopamine degradation.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
<a href="/cs/project/GA14-22426S" target="_blank" >GA14-22426S: Vývoj multifunkční monolitické kapilární kolony s integrovanou úpravou vzorku, separací a elektrochemickou detekcí</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Separation Science
ISSN
1615-9306
e-ISSN
—
Svazek periodika
39
Číslo periodika v rámci svazku
21
Stát vydavatele periodika
DE - Spolková republika Německo
Počet stran výsledku
9
Strana od-do
4107-4115
Kód UT WoS článku
000389046300006
EID výsledku v databázi Scopus
2-s2.0-84994472154