Silver-Ion Liquid Chromatography–Mass Spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F17%3A39910784" target="_blank" >RIV/00216275:25310/17:39910784 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Silver-Ion Liquid Chromatography–Mass Spectrometry

Popis výsledku v původním jazyce

Silver-ion high-performance liquid chromatography (Ag-HPLC) coupled to mass spectrometry provides a unique capability to separate molecules according to the number, positions and geometry of double bonds (DB), which can be applied for characterization of the DB arrangement in triacylglycerols, fatty acid methyl esters, and other unsaturated molecules with low to medium polarity. Ag-HPLC is the most reliable technique for the regioisomeric separation of triacylglycerols. Ag-HPLC provides a high orthogonality to reversed-phase HPLC, therefore various configurations of 2D coupling of silver-ion and reversed phase modes give a higher peak capacity for the comprehensive analysis of glycerolipids. The governing factor for the retention order in Ag-HPLC is the total number of DB, which causes the separation of complex mixtures into distinct groups differing in the DB number. The second factor is the DB geometry, where the retention is significantly lower for trans-isomers and finally the smallest effect on the retention is observed for regioisomers.

Název v anglickém jazyce

Silver-Ion Liquid Chromatography–Mass Spectrometry

Popis výsledku anglicky

Silver-ion high-performance liquid chromatography (Ag-HPLC) coupled to mass spectrometry provides a unique capability to separate molecules according to the number, positions and geometry of double bonds (DB), which can be applied for characterization of the DB arrangement in triacylglycerols, fatty acid methyl esters, and other unsaturated molecules with low to medium polarity. Ag-HPLC is the most reliable technique for the regioisomeric separation of triacylglycerols. Ag-HPLC provides a high orthogonality to reversed-phase HPLC, therefore various configurations of 2D coupling of silver-ion and reversed phase modes give a higher peak capacity for the comprehensive analysis of glycerolipids. The governing factor for the retention order in Ag-HPLC is the total number of DB, which causes the separation of complex mixtures into distinct groups differing in the DB number. The second factor is the DB geometry, where the retention is significantly lower for trans-isomers and finally the smallest effect on the retention is observed for regioisomers.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LL1302" target="_blank" >LL1302: Hmotnostní spektrometrie při hledání lipidových biomarkerů pro včasnou diagnostiku rakoviny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Handbook of Advanced Chromatography /Mass Spectrometry Techniques

ISBN

978-0-12-811732-3

Počet stran výsledku

26

Strana od-do

115-140

Počet stran knihy

504

Název nakladatele

Academic Press (Elsevier)

Místo vydání

Cambridge

Kód UT WoS kapitoly

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