Isotachophoretic Determination of Amino Acids After Their Conversion to Hydroxy Acids

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isotachophoretic Determination of Amino Acids After Their Conversion to Hydroxy Acids

Popis výsledku v původním jazyce

Determination of amino acids by capillary isotachophoresis is presented. Developed method is suitable for separation of amino acids from relatively simple mixtures, especially for dietary supplements. The most of amino acids is not possible to separate directly by isotachophoresis. Thus, they had to be converted to other compounds. Chemical conversion to corresponding hydroxy acids in presence of nitrite acid was used. This necessary nitrite acid was prepared by reaction of sodium nitrite with acetic acid. Under optimization, concentration 0.1 mol/L sodium nitrite and acetic acid was found. As the best electrolyte system for separation of hydroxy acids, 0.01 mol/L HCl, 0.05% hydroxyethyl cellulose, with β-alanine (leading electrolyte of pH=3.6) and 0.01 mol/L valeric acid with sodiumhydroxide (terminating electrolyte of pH=7.24) were chosen.

Název v anglickém jazyce

Isotachophoretic Determination of Amino Acids After Their Conversion to Hydroxy Acids

Popis výsledku anglicky

Determination of amino acids by capillary isotachophoresis is presented. Developed method is suitable for separation of amino acids from relatively simple mixtures, especially for dietary supplements. The most of amino acids is not possible to separate directly by isotachophoresis. Thus, they had to be converted to other compounds. Chemical conversion to corresponding hydroxy acids in presence of nitrite acid was used. This necessary nitrite acid was prepared by reaction of sodium nitrite with acetic acid. Under optimization, concentration 0.1 mol/L sodium nitrite and acetic acid was found. As the best electrolyte system for separation of hydroxy acids, 0.01 mol/L HCl, 0.05% hydroxyethyl cellulose, with β-alanine (leading electrolyte of pH=3.6) and 0.01 mol/L valeric acid with sodiumhydroxide (terminating electrolyte of pH=7.24) were chosen.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

13th ISC Modern Analytical Chemistry

ISBN

978-80-7444-052-6

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

7

Strana od-do

20-26

Název nakladatele

Univerzita Karlova. Přírodovědecká fakulta

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

20. 9. 2017

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000435216500004