Isotachophoretic determination of amino acids after their conversion to hydroxy acids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F18%3A39912912" target="_blank" >RIV/00216275:25310/18:39912912 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00706-018-2220-0" target="_blank" >http://dx.doi.org/10.1007/s00706-018-2220-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-018-2220-0" target="_blank" >10.1007/s00706-018-2220-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Isotachophoretic determination of amino acids after their conversion to hydroxy acids

Popis výsledku v původním jazyce

In this article, determination of amino acids by capillary isotachophoresis based on their derivatization (van Slyke reaction) is presented. Hence, the suggested method deals with reaction of amino acids with nitrous acid forming corresponding hydroxy acids. This reaction was used in isotachophoretic analysis for the first time. Optimal conditions represented by electrolyte system for separation of hydroxy acids, 0.01 mol/dm(3) HCl, 0.05% hydroxyethyl cellulose, with beta-alanine (leading electrolyte of pH 3.6) and 0.01 mol/dm(3) valeric acid with sodium hydroxide (terminating electrolyte of pH 7.24), were chosen; furthermore, other critical details of the analysis are also described. Developed method brings original results and shows applicability on analysis of amino acids in relatively simple mixtures, especially in food supplements. [GRAPHICS] .

Název v anglickém jazyce

Isotachophoretic determination of amino acids after their conversion to hydroxy acids

Popis výsledku anglicky

In this article, determination of amino acids by capillary isotachophoresis based on their derivatization (van Slyke reaction) is presented. Hence, the suggested method deals with reaction of amino acids with nitrous acid forming corresponding hydroxy acids. This reaction was used in isotachophoretic analysis for the first time. Optimal conditions represented by electrolyte system for separation of hydroxy acids, 0.01 mol/dm(3) HCl, 0.05% hydroxyethyl cellulose, with beta-alanine (leading electrolyte of pH 3.6) and 0.01 mol/dm(3) valeric acid with sodium hydroxide (terminating electrolyte of pH 7.24), were chosen; furthermore, other critical details of the analysis are also described. Developed method brings original results and shows applicability on analysis of amino acids in relatively simple mixtures, especially in food supplements. [GRAPHICS] .

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte fur Chemie

ISSN

0026-9247

e-ISSN

—

Svazek periodika

149

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

1709-1718

Kód UT WoS článku

000442501400029

EID výsledku v databázi Scopus

2-s2.0-85051298083