Voltammetric determination of leucovorin in pharmaceutical preparations using a boron-doped diamond electrode

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F18%3A39913160" target="_blank" >RIV/00216275:25310/18:39913160 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388955:_____/18:00492515

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00706-018-2200-4" target="_blank" >http://dx.doi.org/10.1007/s00706-018-2200-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-018-2200-4" target="_blank" >10.1007/s00706-018-2200-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Voltammetric determination of leucovorin in pharmaceutical preparations using a boron-doped diamond electrode

Popis výsledku v původním jazyce

Method for voltammetric determination of leucovorin, a drug frequently applied to decrease some unfavorable effects of anticancer drugs such as methotrexate or to increase the therapeutic effect of 5-fluorouracil, has been developed employing a bare boron-doped diamond electrode. It is the first method for leucovorin determination based on its electrochemical oxidation. Although at least three anodic and three cathodic voltammetric peaks could be recorded under the used conditions, only the anodic response situated at about + 900 mV (vs. saturated Ag|AgCl electrode) was suitable, namely due to its shape and position, for analytical purposes. Using differential pulse voltammetry with optimized parameters and supporting electrolyte of pH 3.0, the linear dynamic range of leucovorin determination was recorded from 0.15 to 25 mu mol dm(-3). Under such conditions, low limit of quantification of 0.050 mu mol dm(-3) and limit of detection of 0.015 mu mol dm(-3) as well was reached. Relative standard deviation calculated from 11 repeated measurements amounted to 0.7% and calculated from five repeated determinations amounting less than 3.0%. Applicability of the developed method was verified by repeated analysis of the pharmaceutical preparation with excellent results (recovery 98.7-102.8%, relative standard deviation 1.81%).

Název v anglickém jazyce

Voltammetric determination of leucovorin in pharmaceutical preparations using a boron-doped diamond electrode

Popis výsledku anglicky

Method for voltammetric determination of leucovorin, a drug frequently applied to decrease some unfavorable effects of anticancer drugs such as methotrexate or to increase the therapeutic effect of 5-fluorouracil, has been developed employing a bare boron-doped diamond electrode. It is the first method for leucovorin determination based on its electrochemical oxidation. Although at least three anodic and three cathodic voltammetric peaks could be recorded under the used conditions, only the anodic response situated at about + 900 mV (vs. saturated Ag|AgCl electrode) was suitable, namely due to its shape and position, for analytical purposes. Using differential pulse voltammetry with optimized parameters and supporting electrolyte of pH 3.0, the linear dynamic range of leucovorin determination was recorded from 0.15 to 25 mu mol dm(-3). Under such conditions, low limit of quantification of 0.050 mu mol dm(-3) and limit of detection of 0.015 mu mol dm(-3) as well was reached. Relative standard deviation calculated from 11 repeated measurements amounted to 0.7% and calculated from five repeated determinations amounting less than 3.0%. Applicability of the developed method was verified by repeated analysis of the pharmaceutical preparation with excellent results (recovery 98.7-102.8%, relative standard deviation 1.81%).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA17-03868S" target="_blank" >GA17-03868S: Nové metody elektrochemického sledování biologicky aktivních organických látek v environmentálních, biologických a potravinových matricích</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte fur Chemie

ISSN

0026-9247

e-ISSN

—

Svazek periodika

149

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

1701-1708

Kód UT WoS článku

000442501400028

EID výsledku v databázi Scopus

2-s2.0-85051284989