Flow injection tyrosinase biosensor for direct determination of acetaminophen in human urine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F19%3A39914601" target="_blank" >RIV/00216275:25310/19:39914601 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s00216-019-01687-4" target="_blank" >https://link.springer.com/article/10.1007/s00216-019-01687-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-019-01687-4" target="_blank" >10.1007/s00216-019-01687-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Flow injection tyrosinase biosensor for direct determination of acetaminophen in human urine

Popis výsledku v původním jazyce

An amperometric biosensor compatible with a flow injection analysis (FIA) for highly selective determination of acetaminophen (APAP) in a sample of human urine was developed. This biosensor is also suitable for use in the routine pharmaceutical practice. To prove this statement, two different commercially available pharmaceutical formulations were analyzed. This nano-(bio)electroanalytical device was made from a commercially available screen-printed carbon electrode covered by a thin layer of non-functionalized graphene (NFG) as amperometric transducer. A biorecognition layer was prepared from mushroom (Agaricus bisporus) tyrosinase (EC 1.14.18.1) cross-linked using glutaraldehyde, where resulting aggregates were covered by Nafion (R), a known ion exchange membrane. Owing to the use of tyrosinase and presence of NFG, the developed analytical instrument is able to measure even at potentials of 0 V. Linear ranges differ according to choice of detection potential, namely up to 130 mu mol L-1 at 0 V, up to 90 mu mol L-1 at -0.1 V, and up to 70 mu mol L-1 at -0.15 V. The first mentioned linear range is described by the equation I-p [mu A] = 0.236-0.1984c [mu mol L-1] and correlation coefficient r = 0.9987; this equation was used to quantify the content of APAP in each sample. The limit of detection of APAP was estimated to be 1.1 mu mol L-1. A recovery of 96.8% (c = 25 mu mol L-1, n = 5 measurements) was calculated. The obtained results show that FIA is a very selective method for APAP determination, being comparable to the chosen reference method of reversed-phase high-performance liquid chromatography.

Název v anglickém jazyce

Flow injection tyrosinase biosensor for direct determination of acetaminophen in human urine

Popis výsledku anglicky

An amperometric biosensor compatible with a flow injection analysis (FIA) for highly selective determination of acetaminophen (APAP) in a sample of human urine was developed. This biosensor is also suitable for use in the routine pharmaceutical practice. To prove this statement, two different commercially available pharmaceutical formulations were analyzed. This nano-(bio)electroanalytical device was made from a commercially available screen-printed carbon electrode covered by a thin layer of non-functionalized graphene (NFG) as amperometric transducer. A biorecognition layer was prepared from mushroom (Agaricus bisporus) tyrosinase (EC 1.14.18.1) cross-linked using glutaraldehyde, where resulting aggregates were covered by Nafion (R), a known ion exchange membrane. Owing to the use of tyrosinase and presence of NFG, the developed analytical instrument is able to measure even at potentials of 0 V. Linear ranges differ according to choice of detection potential, namely up to 130 mu mol L-1 at 0 V, up to 90 mu mol L-1 at -0.1 V, and up to 70 mu mol L-1 at -0.15 V. The first mentioned linear range is described by the equation I-p [mu A] = 0.236-0.1984c [mu mol L-1] and correlation coefficient r = 0.9987; this equation was used to quantify the content of APAP in each sample. The limit of detection of APAP was estimated to be 1.1 mu mol L-1. A recovery of 96.8% (c = 25 mu mol L-1, n = 5 measurements) was calculated. The obtained results show that FIA is a very selective method for APAP determination, being comparable to the chosen reference method of reversed-phase high-performance liquid chromatography.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

—

Svazek periodika

411

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

2415-2424

Kód UT WoS článku

000464715500016

EID výsledku v databázi Scopus

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