IN VITRO ACTIVITY OF SPICE EXTRACTS AGAINST ARCOBACTER SPP. AND INFLUENCE ON THEIR BIOFILM FORMATION

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F19%3A39914721" target="_blank" >RIV/00216275:25310/19:39914721 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.jmbfs.org/wp-content/uploads/2019/11/jmbfs_1272_Silha.pdf" target="_blank" >https://www.jmbfs.org/wp-content/uploads/2019/11/jmbfs_1272_Silha.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.15414/jmbfs.2019/20.9.3.552-556" target="_blank" >10.15414/jmbfs.2019/20.9.3.552-556</a>

Jazyk výsledku

angličtina

Název v původním jazyce

IN VITRO ACTIVITY OF SPICE EXTRACTS AGAINST ARCOBACTER SPP. AND INFLUENCE ON THEIR BIOFILM FORMATION

Popis výsledku v původním jazyce

Arcobacter spp. are dangerous bacteria with biofilm formation ability. The plant materials with antimicrobial potential are required. The antimicrobial effect of several plant extracts were examined by a disk diffusion method. The effect of extracts on biofilm formation was monitored using Christensen method. Tests were carried out with much less known and studied arcobacters as well. Arcobacters were highly resistant to extracts from juniper, nutmeg and anise. Minimum inhibitory concentrations (MIC) of MetOH/DMSO (extraction in methanol/final dissolution in dimethylsulfoxide) of cloves, nutmeg and star anise extract were determined after 24 hours of exposure. The clove MetOH/DMSO extract had a MIC≥20 mg/mL, nutmeg MetOH/DMSO extract had MIC 10-20 mg/mL, and the highest values were recorded in star anise extract (MIC≥20 mg/mL). Furthermore, the tested extracts were found to have a dual effect on biofilm formation. On the one hand, the elimination of biofilm formation was observed, but also its stimulation, depending on the type of extract and the bacterial strain. The results indicate a particularly interesting antimicrobial effect of cloves and cinnamon extract (inhibition zones up to 30 mm) against Arcobacter strains. The study shows a promising effect of tested extracts on biofilm formation for the first time.

Název v anglickém jazyce

IN VITRO ACTIVITY OF SPICE EXTRACTS AGAINST ARCOBACTER SPP. AND INFLUENCE ON THEIR BIOFILM FORMATION

Popis výsledku anglicky

Arcobacter spp. are dangerous bacteria with biofilm formation ability. The plant materials with antimicrobial potential are required. The antimicrobial effect of several plant extracts were examined by a disk diffusion method. The effect of extracts on biofilm formation was monitored using Christensen method. Tests were carried out with much less known and studied arcobacters as well. Arcobacters were highly resistant to extracts from juniper, nutmeg and anise. Minimum inhibitory concentrations (MIC) of MetOH/DMSO (extraction in methanol/final dissolution in dimethylsulfoxide) of cloves, nutmeg and star anise extract were determined after 24 hours of exposure. The clove MetOH/DMSO extract had a MIC≥20 mg/mL, nutmeg MetOH/DMSO extract had MIC 10-20 mg/mL, and the highest values were recorded in star anise extract (MIC≥20 mg/mL). Furthermore, the tested extracts were found to have a dual effect on biofilm formation. On the one hand, the elimination of biofilm formation was observed, but also its stimulation, depending on the type of extract and the bacterial strain. The results indicate a particularly interesting antimicrobial effect of cloves and cinnamon extract (inhibition zones up to 30 mm) against Arcobacter strains. The study shows a promising effect of tested extracts on biofilm formation for the first time.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Microbiology, Biotechnology and Food Sciences

ISSN

1338-5178

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

5

Strana od-do

552-556

Kód UT WoS článku

000500542200012

EID výsledku v databázi Scopus

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