Colloidally stable polypeptide-based nanogel: Study of enzyme-mediated nanogelation in inverse miniemulsion
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F19%3A39914952" target="_blank" >RIV/00216275:25310/19:39914952 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61389013:_____/20:00522680
Výsledek na webu
<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1002/app.48725" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1002/app.48725</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/app.48725" target="_blank" >10.1002/app.48725</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Colloidally stable polypeptide-based nanogel: Study of enzyme-mediated nanogelation in inverse miniemulsion
Popis výsledku v původním jazyce
The current work presents a pivotal study of the nanogelation of the linear poly(N-5-2-hydroxypropyl-L-glutamine) polymer precursor containing tyramine (TYR) units in an inverse miniemulsion by horseradish peroxidase/H2O2-mediated crosslinking. The effects of various n(H2O2)/n(TYR) ratios on the kinetics of nanogelation in the inverse miniemulsion and on the reaction time are investigated by linear sweep voltammetry, while the formation of dityramine crosslinking is explored by fluorescence spectroscopy. The study is completed using dynamic light scattering measurements, nanoparticle tracking analysis, and cryogenic transmission electron microscopy to acquire comprehensive information about the formed nanoparticulate systems. With the optimal ratio n(H2O2)/n(TYR) = 2, the strategy yields in the high-quality 130 nm poly(amino acid)-based nanogel, which is prepared in 2 h. The nanogel is colloidally stable under different temperature and pH conditions for over 168 h. Moreover, the demonstrated nanogel is noncytotoxic for HeLa cells and human primary fibroblasts and is quickly enzymatically hydrolyzed into small fragments during a biodegradation study in human blood plasma. (c) 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 137, 48725.
Název v anglickém jazyce
Colloidally stable polypeptide-based nanogel: Study of enzyme-mediated nanogelation in inverse miniemulsion
Popis výsledku anglicky
The current work presents a pivotal study of the nanogelation of the linear poly(N-5-2-hydroxypropyl-L-glutamine) polymer precursor containing tyramine (TYR) units in an inverse miniemulsion by horseradish peroxidase/H2O2-mediated crosslinking. The effects of various n(H2O2)/n(TYR) ratios on the kinetics of nanogelation in the inverse miniemulsion and on the reaction time are investigated by linear sweep voltammetry, while the formation of dityramine crosslinking is explored by fluorescence spectroscopy. The study is completed using dynamic light scattering measurements, nanoparticle tracking analysis, and cryogenic transmission electron microscopy to acquire comprehensive information about the formed nanoparticulate systems. With the optimal ratio n(H2O2)/n(TYR) = 2, the strategy yields in the high-quality 130 nm poly(amino acid)-based nanogel, which is prepared in 2 h. The nanogel is colloidally stable under different temperature and pH conditions for over 168 h. Moreover, the demonstrated nanogel is noncytotoxic for HeLa cells and human primary fibroblasts and is quickly enzymatically hydrolyzed into small fragments during a biodegradation study in human blood plasma. (c) 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 137, 48725.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
21001 - Nano-materials (production and properties)
Návaznosti výsledku
Projekt
<a href="/cs/project/GA18-03224S" target="_blank" >GA18-03224S: INJIKOVATELNÉ HYDROGELY NA BÁZI POLYPEPTIDŮ NAPODOBUJÍCÍ DYNAMICKÉ PROCESY MEZIBUNĚČNÉ HMOTY</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Applied Polymer Science
ISSN
0021-8995
e-ISSN
—
Svazek periodika
137
Číslo periodika v rámci svazku
21
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
10
Strana od-do
"48725-1"-"48725-10"
Kód UT WoS článku
000496555800001
EID výsledku v databázi Scopus
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