Poly(amino acid)-based nanogel by horseradish peroxidase catalyzed crosslinking in an inverse miniemulsion
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F18%3A00489472" target="_blank" >RIV/61389013:_____/18:00489472 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1007/s00396-018-4318-7" target="_blank" >http://dx.doi.org/10.1007/s00396-018-4318-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00396-018-4318-7" target="_blank" >10.1007/s00396-018-4318-7</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Poly(amino acid)-based nanogel by horseradish peroxidase catalyzed crosslinking in an inverse miniemulsion
Popis výsledku v původním jazyce
We present an investigation of horseradish peroxidase (HRP)/H2O2-mediated crosslinking in an inverse miniemulsion for the successful preparation of a stable colloidal nanogel from a poly(amino acid)-based polymer precursor. The precursor was obtained by the aminolysis of polysuccinimide with aminoethan-2-ol and tyramine, resulting in a poly(α,β-N-(2-hydroxyethyl)-D,L-aspartamide-co-N-(2-(4-hydroxyphenyl)ethyl)-D,L-aspartamide) polymer (PHEA-Tyr). Various concentrations of the PHEA-Tyr in aqueous solution with HRP were emulsified in the presence of cyclohexane and SPAN 80. The addition of a hydrogen peroxide solution induced crosslinking between the polymer chains via the phenol groups (Tyr) and targeted nanogel formation. The hydrodynamic radii (R h 0), mean size documented by hydrodynamic radius (R h ), and morphology of the nanoparticles were investigated by dynamic light scattering (DLS) measurements, nanoparticle tracking analysis (NTA), and cryogenic transmission electron microscopy (cryo-TEM). It was found out that nanoparticle radius, morphology, and architecture of the nanogel could be regulated by the initial concentration of the precursor.
Název v anglickém jazyce
Poly(amino acid)-based nanogel by horseradish peroxidase catalyzed crosslinking in an inverse miniemulsion
Popis výsledku anglicky
We present an investigation of horseradish peroxidase (HRP)/H2O2-mediated crosslinking in an inverse miniemulsion for the successful preparation of a stable colloidal nanogel from a poly(amino acid)-based polymer precursor. The precursor was obtained by the aminolysis of polysuccinimide with aminoethan-2-ol and tyramine, resulting in a poly(α,β-N-(2-hydroxyethyl)-D,L-aspartamide-co-N-(2-(4-hydroxyphenyl)ethyl)-D,L-aspartamide) polymer (PHEA-Tyr). Various concentrations of the PHEA-Tyr in aqueous solution with HRP were emulsified in the presence of cyclohexane and SPAN 80. The addition of a hydrogen peroxide solution induced crosslinking between the polymer chains via the phenol groups (Tyr) and targeted nanogel formation. The hydrodynamic radii (R h 0), mean size documented by hydrodynamic radius (R h ), and morphology of the nanoparticles were investigated by dynamic light scattering (DLS) measurements, nanoparticle tracking analysis (NTA), and cryogenic transmission electron microscopy (cryo-TEM). It was found out that nanoparticle radius, morphology, and architecture of the nanogel could be regulated by the initial concentration of the precursor.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10404 - Polymer science
Návaznosti výsledku
Projekt
<a href="/cs/project/GA16-02702S" target="_blank" >GA16-02702S: Materiály s nanostrukturovaným povrchem pro biomediciální aplikace</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2018
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Colloid and Polymer Science
ISSN
0303-402X
e-ISSN
—
Svazek periodika
296
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
DE - Spolková republika Německo
Počet stran výsledku
9
Strana od-do
995-1003
Kód UT WoS článku
000431929900001
EID výsledku v databázi Scopus
2-s2.0-85046862434