Poly(amino acid)-based nanogel by horseradish peroxidase catalyzed crosslinking in an inverse miniemulsion

Kód výsledku v IS VaVaI

RIV/61389013:_____/18:00489472 - isvavai.cz

Výsledek na webu

http://dx.doi.org/10.1007/s00396-018-4318-7

DOI - Digital Object Identifier

10.1007/s00396-018-4318-7

Jazyk výsledku

angličtina

Název v původním jazyce

Poly(amino acid)-based nanogel by horseradish peroxidase catalyzed crosslinking in an inverse miniemulsion

Popis výsledku v původním jazyce

We present an investigation of horseradish peroxidase (HRP)/H2O2-mediated crosslinking in an inverse miniemulsion for the successful preparation of a stable colloidal nanogel from a poly(amino acid)-based polymer precursor. The precursor was obtained by the aminolysis of polysuccinimide with aminoethan-2-ol and tyramine, resulting in a poly(α,β-N-(2-hydroxyethyl)-D,L-aspartamide-co-N-(2-(4-hydroxyphenyl)ethyl)-D,L-aspartamide) polymer (PHEA-Tyr). Various concentrations of the PHEA-Tyr in aqueous solution with HRP were emulsified in the presence of cyclohexane and SPAN 80. The addition of a hydrogen peroxide solution induced crosslinking between the polymer chains via the phenol groups (Tyr) and targeted nanogel formation. The hydrodynamic radii (R h 0), mean size documented by hydrodynamic radius (R h ), and morphology of the nanoparticles were investigated by dynamic light scattering (DLS) measurements, nanoparticle tracking analysis (NTA), and cryogenic transmission electron microscopy (cryo-TEM). It was found out that nanoparticle radius, morphology, and architecture of the nanogel could be regulated by the initial concentration of the precursor.

Název v anglickém jazyce

Poly(amino acid)-based nanogel by horseradish peroxidase catalyzed crosslinking in an inverse miniemulsion

Popis výsledku anglicky

We present an investigation of horseradish peroxidase (HRP)/H2O2-mediated crosslinking in an inverse miniemulsion for the successful preparation of a stable colloidal nanogel from a poly(amino acid)-based polymer precursor. The precursor was obtained by the aminolysis of polysuccinimide with aminoethan-2-ol and tyramine, resulting in a poly(α,β-N-(2-hydroxyethyl)-D,L-aspartamide-co-N-(2-(4-hydroxyphenyl)ethyl)-D,L-aspartamide) polymer (PHEA-Tyr). Various concentrations of the PHEA-Tyr in aqueous solution with HRP were emulsified in the presence of cyclohexane and SPAN 80. The addition of a hydrogen peroxide solution induced crosslinking between the polymer chains via the phenol groups (Tyr) and targeted nanogel formation. The hydrodynamic radii (R h 0), mean size documented by hydrodynamic radius (R h ), and morphology of the nanoparticles were investigated by dynamic light scattering (DLS) measurements, nanoparticle tracking analysis (NTA), and cryogenic transmission electron microscopy (cryo-TEM). It was found out that nanoparticle radius, morphology, and architecture of the nanogel could be regulated by the initial concentration of the precursor.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

GA16-02702S: Materiály s nanostrukturovaným povrchem pro biomediciální aplikace

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Colloid and Polymer Science

ISSN

0303-402X

e-ISSN

—

Svazek periodika

296

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

995-1003

Kód UT WoS článku

000431929900001

EID výsledku v databázi Scopus

2-s2.0-85046862434