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Dried blood spot as an alternative sample for screening of fatty acid, amino acid, and keto acid metabolism in humans

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F22%3A39919063" target="_blank" >RIV/00216275:25310/22:39919063 - isvavai.cz</a>

  • Výsledek na webu

    <a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/bmc.5431" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/bmc.5431</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/bmc.5431" target="_blank" >10.1002/bmc.5431</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Dried blood spot as an alternative sample for screening of fatty acid, amino acid, and keto acid metabolism in humans

  • Popis výsledku v původním jazyce

    Dried blood spot (DBS) is a simple and noninvasive sample collection technique allowing self-collection at home. It can be used as an alternative sample for the screening of metabolism in humans because changes in the levels of some fatty acids (FAs), amino acids (AAs), and keto acids (KAs) can be associated with metabolic disorders (e.g., diabetes mellitus). In this study, we optimized three different methods that are sensitive enough for the determination of the aforementioned analytes from a small volume of biological material in DBS. A total of 20 AAs, 5 KAs, and 24 FAs were determined. This sampling technique was applied to prepare samples from 60 individuals by a finger prick. The samples were analyzed using chromatographic methods, and acquired data were statistically evaluated. Even though most analytes were higher in men, only five AAs, three KAs, and eight FAs showed significant gender dependency (alpha = 0.05). Asparagine, serine, and alpha- and gamma-linolenic acids showed significant age dependency (alpha = 0.05). Most statistically significant correlations were positive and were found within one category. This work shows that because of many benefits, the DBS sample could be a good alternative to whole blood sample collection for the screening of metabolism in humans, in general, or in individualized medicine. The chromatographic methods can be used in future research, for example, to set the reference range or plasma-correction factors (various aspects such as age or gender should be considered).

  • Název v anglickém jazyce

    Dried blood spot as an alternative sample for screening of fatty acid, amino acid, and keto acid metabolism in humans

  • Popis výsledku anglicky

    Dried blood spot (DBS) is a simple and noninvasive sample collection technique allowing self-collection at home. It can be used as an alternative sample for the screening of metabolism in humans because changes in the levels of some fatty acids (FAs), amino acids (AAs), and keto acids (KAs) can be associated with metabolic disorders (e.g., diabetes mellitus). In this study, we optimized three different methods that are sensitive enough for the determination of the aforementioned analytes from a small volume of biological material in DBS. A total of 20 AAs, 5 KAs, and 24 FAs were determined. This sampling technique was applied to prepare samples from 60 individuals by a finger prick. The samples were analyzed using chromatographic methods, and acquired data were statistically evaluated. Even though most analytes were higher in men, only five AAs, three KAs, and eight FAs showed significant gender dependency (alpha = 0.05). Asparagine, serine, and alpha- and gamma-linolenic acids showed significant age dependency (alpha = 0.05). Most statistically significant correlations were positive and were found within one category. This work shows that because of many benefits, the DBS sample could be a good alternative to whole blood sample collection for the screening of metabolism in humans, in general, or in individualized medicine. The chromatographic methods can be used in future research, for example, to set the reference range or plasma-correction factors (various aspects such as age or gender should be considered).

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    10406 - Analytical chemistry

Návaznosti výsledku

  • Projekt

  • Návaznosti

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2022

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Biomedical Chromatography

  • ISSN

    0269-3879

  • e-ISSN

    1099-0801

  • Svazek periodika

    36

  • Číslo periodika v rámci svazku

    9

  • Stát vydavatele periodika

    GB - Spojené království Velké Británie a Severního Irska

  • Počet stran výsledku

    15

  • Strana od-do

    "e5431"

  • Kód UT WoS článku

    000819644100001

  • EID výsledku v databázi Scopus

    2-s2.0-85133153050