Dried blood spot as an alternative sample for screening of fatty acid, amino acid, and keto acid metabolism in humans
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F22%3A39919063" target="_blank" >RIV/00216275:25310/22:39919063 - isvavai.cz</a>
Výsledek na webu
<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/bmc.5431" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/bmc.5431</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/bmc.5431" target="_blank" >10.1002/bmc.5431</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Dried blood spot as an alternative sample for screening of fatty acid, amino acid, and keto acid metabolism in humans
Popis výsledku v původním jazyce
Dried blood spot (DBS) is a simple and noninvasive sample collection technique allowing self-collection at home. It can be used as an alternative sample for the screening of metabolism in humans because changes in the levels of some fatty acids (FAs), amino acids (AAs), and keto acids (KAs) can be associated with metabolic disorders (e.g., diabetes mellitus). In this study, we optimized three different methods that are sensitive enough for the determination of the aforementioned analytes from a small volume of biological material in DBS. A total of 20 AAs, 5 KAs, and 24 FAs were determined. This sampling technique was applied to prepare samples from 60 individuals by a finger prick. The samples were analyzed using chromatographic methods, and acquired data were statistically evaluated. Even though most analytes were higher in men, only five AAs, three KAs, and eight FAs showed significant gender dependency (alpha = 0.05). Asparagine, serine, and alpha- and gamma-linolenic acids showed significant age dependency (alpha = 0.05). Most statistically significant correlations were positive and were found within one category. This work shows that because of many benefits, the DBS sample could be a good alternative to whole blood sample collection for the screening of metabolism in humans, in general, or in individualized medicine. The chromatographic methods can be used in future research, for example, to set the reference range or plasma-correction factors (various aspects such as age or gender should be considered).
Název v anglickém jazyce
Dried blood spot as an alternative sample for screening of fatty acid, amino acid, and keto acid metabolism in humans
Popis výsledku anglicky
Dried blood spot (DBS) is a simple and noninvasive sample collection technique allowing self-collection at home. It can be used as an alternative sample for the screening of metabolism in humans because changes in the levels of some fatty acids (FAs), amino acids (AAs), and keto acids (KAs) can be associated with metabolic disorders (e.g., diabetes mellitus). In this study, we optimized three different methods that are sensitive enough for the determination of the aforementioned analytes from a small volume of biological material in DBS. A total of 20 AAs, 5 KAs, and 24 FAs were determined. This sampling technique was applied to prepare samples from 60 individuals by a finger prick. The samples were analyzed using chromatographic methods, and acquired data were statistically evaluated. Even though most analytes were higher in men, only five AAs, three KAs, and eight FAs showed significant gender dependency (alpha = 0.05). Asparagine, serine, and alpha- and gamma-linolenic acids showed significant age dependency (alpha = 0.05). Most statistically significant correlations were positive and were found within one category. This work shows that because of many benefits, the DBS sample could be a good alternative to whole blood sample collection for the screening of metabolism in humans, in general, or in individualized medicine. The chromatographic methods can be used in future research, for example, to set the reference range or plasma-correction factors (various aspects such as age or gender should be considered).
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Biomedical Chromatography
ISSN
0269-3879
e-ISSN
1099-0801
Svazek periodika
36
Číslo periodika v rámci svazku
9
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
15
Strana od-do
"e5431"
Kód UT WoS článku
000819644100001
EID výsledku v databázi Scopus
2-s2.0-85133153050