The Influence of Proteins and Speed on Friction and Adsorption of Metal/UHMWPE Contact Pair

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26210%2F17%3APU123529" target="_blank" >RIV/00216305:26210/17:PU123529 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S2352573816300749" target="_blank" >http://www.sciencedirect.com/science/article/pii/S2352573816300749</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biotri.2017.03.003" target="_blank" >10.1016/j.biotri.2017.03.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Influence of Proteins and Speed on Friction and Adsorption of Metal/UHMWPE Contact Pair

Popis výsledku v původním jazyce

It was reported in several studies that friction and wear of joint prosthesis are apparently influenced by the proteins contained in the synovial fluid. However, detailed mechanisms of these tribological processes have not been clarified yet. The present study aims on the effect of adsorbed protein film on frictional behaviour of metal/polyethylene contact pair. Reciprocating pin-on-plate test was conducted, while the CoCrMo pin was sliding against UHMWPE plate. The contact was lubricated by various solutions of albumin and γ-globulin solved in PBS. After the friction test, the thickness of adsorbed film was evaluated using spectroscopic ellipsometry. Structure of the adsorbed proteins was later examined by FT-IR. The results showed that at low sliding speed equal to 10 mm/s, there was a linear correlation between the friction coefficient and the thickness of the adsorbed protein film. An increase of friction was thus accompanied by the evolution of protein film. In that case, both proteins undergone substantial conformational changes, losing their original structure. On the contrary, proteins could sustain their secondary structure to some extent at higher sliding speed (50 mm/s), when different behaviour of the both proteins could be observed. This phenomenon was attributed to different structure of albumin and γ-globulin in its native state. It might be concluded that friction coefficient of metal-on-polyethylene joint prosthesis is influenced by protein content, as well as kinematic conditions, since the sliding conditions have a certain effect on both the adsorbed film formation and structure of the adsorbed molecules.

Název v anglickém jazyce

The Influence of Proteins and Speed on Friction and Adsorption of Metal/UHMWPE Contact Pair

Popis výsledku anglicky

It was reported in several studies that friction and wear of joint prosthesis are apparently influenced by the proteins contained in the synovial fluid. However, detailed mechanisms of these tribological processes have not been clarified yet. The present study aims on the effect of adsorbed protein film on frictional behaviour of metal/polyethylene contact pair. Reciprocating pin-on-plate test was conducted, while the CoCrMo pin was sliding against UHMWPE plate. The contact was lubricated by various solutions of albumin and γ-globulin solved in PBS. After the friction test, the thickness of adsorbed film was evaluated using spectroscopic ellipsometry. Structure of the adsorbed proteins was later examined by FT-IR. The results showed that at low sliding speed equal to 10 mm/s, there was a linear correlation between the friction coefficient and the thickness of the adsorbed protein film. An increase of friction was thus accompanied by the evolution of protein film. In that case, both proteins undergone substantial conformational changes, losing their original structure. On the contrary, proteins could sustain their secondary structure to some extent at higher sliding speed (50 mm/s), when different behaviour of the both proteins could be observed. This phenomenon was attributed to different structure of albumin and γ-globulin in its native state. It might be concluded that friction coefficient of metal-on-polyethylene joint prosthesis is influenced by protein content, as well as kinematic conditions, since the sliding conditions have a certain effect on both the adsorbed film formation and structure of the adsorbed molecules.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

20301 - Mechanical engineering

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biotribology

ISSN

2352-5738

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

2017

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

51-59

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85018920034