Studium možnosti separace thaumatinu kapilární elektroforézou

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F03%3APU49867" target="_blank" >RIV/00216305:26310/03:PU49867 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Exploration on the separability of thaumatin by capillary electrophoresis

Popis výsledku v původním jazyce

Electrophoretic behaviour of highly basic protein thaumatin was explored in strongly acid (pH 2) and mildly acid (pH 4.5) separation systems using both bare and coated fused silica capillaries. Separation selectivity for thaumatin I, thaumatin II and forother sample constituents was nor sufficient for their baseline separation at pH 2 with uncoated capillary because of separation efficiency, which was markedly lower than common in electrophoretic separations of proteins. Separation selectivity higher uup to one order of magnitude has been reached at pH 4.5. Pronounced asymmetry of zones, which deteriorated resolution at this pH, was effectively suppressed by the coating of the capillary wall with a polymer. Electrochromatographic separation systems based on capillaries coated with the layer of either cationic or hydrophilic uncharged polymer appear to be promising for the development of methods for thaumatin analysis.

Název v anglickém jazyce

Exploration on the separability of thaumatin by capillary electrophoresis

Popis výsledku anglicky

Electrophoretic behaviour of highly basic protein thaumatin was explored in strongly acid (pH 2) and mildly acid (pH 4.5) separation systems using both bare and coated fused silica capillaries. Separation selectivity for thaumatin I, thaumatin II and forother sample constituents was nor sufficient for their baseline separation at pH 2 with uncoated capillary because of separation efficiency, which was markedly lower than common in electrophoretic separations of proteins. Separation selectivity higher uup to one order of magnitude has been reached at pH 4.5. Pronounced asymmetry of zones, which deteriorated resolution at this pH, was effectively suppressed by the coating of the capillary wall with a polymer. Electrochromatographic separation systems based on capillaries coated with the layer of either cationic or hydrophilic uncharged polymer appear to be promising for the development of methods for thaumatin analysis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

26

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

727-733

Kód UT WoS článku

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EID výsledku v databázi Scopus

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