The application of PCR methods and gas chromatography for detection of specific non-pathogenic bacteria of the genus Clostridium

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F09%3APU87649" target="_blank" >RIV/00216305:26310/09:PU87649 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The application of PCR methods and gas chromatography for detection of specific non-pathogenic bacteria of the genus Clostridium

Popis výsledku v původním jazyce

The aim of this study was to select and investigate non-pathogenic bacterial strains of the genus Clostridium collected in the Dairy Research Institute. The strains were isolated from cheeses with late blowing disorders. First, a set of 44 strains was identified into the genus Clostridium using genus-specific PCR (Rekha et al., 2006). PCRs specific to the species Clostridium butyricum (Nakanishi et al., 2005) and Clostridium tyrobutyricum (Herman et al., 1995) were used for species identification. It was found that 7 and 12 strains belonged to the species C. butyricum or C. tyrobutyricum, respectively. Some strains were not identified into species yet. Strains encoding the H2-evolving hydrogenase (hydA) gene were also identified using PCR ( ). Formation of fatty acids (acetic, propionic, and butyric) was studied by gas chromatography in culture supernatants.

Název v anglickém jazyce

The application of PCR methods and gas chromatography for detection of specific non-pathogenic bacteria of the genus Clostridium

Popis výsledku anglicky

The aim of this study was to select and investigate non-pathogenic bacterial strains of the genus Clostridium collected in the Dairy Research Institute. The strains were isolated from cheeses with late blowing disorders. First, a set of 44 strains was identified into the genus Clostridium using genus-specific PCR (Rekha et al., 2006). PCRs specific to the species Clostridium butyricum (Nakanishi et al., 2005) and Clostridium tyrobutyricum (Herman et al., 1995) were used for species identification. It was found that 7 and 12 strains belonged to the species C. butyricum or C. tyrobutyricum, respectively. Some strains were not identified into species yet. Strains encoding the H2-evolving hydrogenase (hydA) gene were also identified using PCR ( ). Formation of fatty acids (acetic, propionic, and butyric) was studied by gas chromatography in culture supernatants.

Druh

O - Ostatní výsledky

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/2B08070" target="_blank" >2B08070: Vývoj nového moderního biotechnologického postupu umožňujícího účinnější způsob využívání odpadních vod z mlékárenského průmyslu jako materiálového zdroje pro výrobu vodíku</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů