Screening of Clostridium strains isolated from cheeses for species identification and formation of fatty acids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F10%3APU89457" target="_blank" >RIV/00216305:26310/10:PU89457 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Screening of Clostridium strains isolated from cheeses for species identification and formation of fatty acids

Popis výsledku v původním jazyce

Non-pathogenic bacterial strains of the Clostridium genus collected in the Dairy Research Institute, Czech Republic, were isolated from cheeses with late blowing disorders. A set of 44 strains was identified into the Clostridium genus using genus-specific PCR. PCRs specific to the species Clostridium butyricum and Clostridium tyrobutyricum were used for species identification. It was found that 7 and 12 strains belonged to the species C. butyricum or C. tyrobutyricum, respectively. Formation of fatty acids (acetic, propionic, and butyric) was studied by capillary isotachophoresis in the cultivation medium. The content of fatty acids was correlated with changes in the cultivation medium's electrical conductance measured with the Rapid Automatic Bacterial Impedance Technique (RABIT). The molecular parameters received were used for correlation with hydrogen production.

Název v anglickém jazyce

Screening of Clostridium strains isolated from cheeses for species identification and formation of fatty acids

Popis výsledku anglicky

Non-pathogenic bacterial strains of the Clostridium genus collected in the Dairy Research Institute, Czech Republic, were isolated from cheeses with late blowing disorders. A set of 44 strains was identified into the Clostridium genus using genus-specific PCR. PCRs specific to the species Clostridium butyricum and Clostridium tyrobutyricum were used for species identification. It was found that 7 and 12 strains belonged to the species C. butyricum or C. tyrobutyricum, respectively. Formation of fatty acids (acetic, propionic, and butyric) was studied by capillary isotachophoresis in the cultivation medium. The content of fatty acids was correlated with changes in the cultivation medium's electrical conductance measured with the Rapid Automatic Bacterial Impedance Technique (RABIT). The molecular parameters received were used for correlation with hydrogen production.

Druh

O - Ostatní výsledky

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/2B08070" target="_blank" >2B08070: Vývoj nového moderního biotechnologického postupu umožňujícího účinnější způsob využívání odpadních vod z mlékárenského průmyslu jako materiálového zdroje pro výrobu vodíku</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů