Characterisation of potential probiotic Lactobacillus strains of human origin
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F10%3APU93667" target="_blank" >RIV/00216305:26310/10:PU93667 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Characterisation of potential probiotic Lactobacillus strains of human origin
Popis výsledku v původním jazyce
Molecular biological methods (genus-specific PCR, species-specific PCRs, RAPD with primer M13, and rep-PCR with primer (GTG)5) were applied for the identification and discrimination of 31 strains of the Lactobacillus genus isolated from faecal samples of30 breastfed infants. All strains were tested for bile salts and low pH tolerance, growth in milk, production of lactic acids in milk supplemented with yeast extract, and used in biochemical tests including production of beta-glucuronidase. Consequently, the presence of genes coding for decarboxylases was investigated using PCR. Finally, four strains of probiotic species were selected for use in the dairy industry and for other tests concerning their probiotic character.
Název v anglickém jazyce
Characterisation of potential probiotic Lactobacillus strains of human origin
Popis výsledku anglicky
Molecular biological methods (genus-specific PCR, species-specific PCRs, RAPD with primer M13, and rep-PCR with primer (GTG)5) were applied for the identification and discrimination of 31 strains of the Lactobacillus genus isolated from faecal samples of30 breastfed infants. All strains were tested for bile salts and low pH tolerance, growth in milk, production of lactic acids in milk supplemented with yeast extract, and used in biochemical tests including production of beta-glucuronidase. Consequently, the presence of genes coding for decarboxylases was investigated using PCR. Finally, four strains of probiotic species were selected for use in the dairy industry and for other tests concerning their probiotic character.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2010
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů