DNA based identification of microorganisms in a probiotic products
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F13%3APU103837" target="_blank" >RIV/00216305:26310/13:PU103837 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
DNA based identification of microorganisms in a probiotic products
Popis výsledku v původním jazyce
Brewers yeasts (Saccharomyces cerevisiae) are a secondary product from production of beer. They are used as a nutritional supplement for long time, too. Brewers yeasts can be supplemented with probiotic bacteria and roughage and with vitamins, minerals and enzymes, too. Brewers yeast tablets with content of genus Lactobacillus and Biffidobacterium were used for analysis. Two types of samples were before expiration, and other were after expiration (1 and 8 years). DNA was isolated from crude cell lysatesof brewers yeast tablets using magnetics P(HEMA-co-GMA) microspheres covered by carboxyl groups. Non-diluted DNA was used for analysis of Lactobacillus and Biffidobacterium. The conventional polymerase chain reaction (PCR) and quantitative real-time PCR(qPCR) were used for amplification of DNAs.
Název v anglickém jazyce
DNA based identification of microorganisms in a probiotic products
Popis výsledku anglicky
Brewers yeasts (Saccharomyces cerevisiae) are a secondary product from production of beer. They are used as a nutritional supplement for long time, too. Brewers yeasts can be supplemented with probiotic bacteria and roughage and with vitamins, minerals and enzymes, too. Brewers yeast tablets with content of genus Lactobacillus and Biffidobacterium were used for analysis. Two types of samples were before expiration, and other were after expiration (1 and 8 years). DNA was isolated from crude cell lysatesof brewers yeast tablets using magnetics P(HEMA-co-GMA) microspheres covered by carboxyl groups. Non-diluted DNA was used for analysis of Lactobacillus and Biffidobacterium. The conventional polymerase chain reaction (PCR) and quantitative real-time PCR(qPCR) were used for amplification of DNAs.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GP13-32840P" target="_blank" >GP13-32840P: Vývoj metody pro studium enkapsulace magnetických nano- a mikročástic pomocí polymerázové řetězové reakce v reálném čase</a><br>
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů