ANALYSIS OF PLASMID DNA ISOLATED FROM STRAINS OF STAPHYLOCOCCUS AUREUS USING ARTIFICIAL NUCLEASES

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F13%3APU104111" target="_blank" >RIV/00216305:26310/13:PU104111 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

ANALYSIS OF PLASMID DNA ISOLATED FROM STRAINS OF STAPHYLOCOCCUS AUREUS USING ARTIFICIAL NUCLEASES

Popis výsledku v původním jazyce

Hydrolysis of nucleic acids mediated by artificial nucleases is very important goal in molecular biology, biotechnology and medicine. Some metal and lanthanide ions and their soluble or insoluble complexes (immobilized on solid particles) are attractiveas catalysts for DNA cleavage. The aim of this work was to use of neodymium ions for cleavage of plasmid DNAs. Plasmid DNAs originated from strains of Staphylococcus aureus isolated from clinical and food samples were treated by neodymium ions (Nd3+). Plasmid pUC19 was used as model molecule and linearized pUC19/EcoRI was used as the control of the presence of linear form. Cleavage of plasmid DNA was carried out at different temperatures in HEPES (0.05M) buffer. The reaction time was up to 4 hours. Thereaction was stopped with 0.5 M EDTA (pH 8.0). Plasmid DNA cleavage efficiency was evaluated by convert the supercoiled circular (ccc) form of plasmid DNA into nicked circular form (oc) and linear (lin) one and was monitored on the gel ac

Název v anglickém jazyce

ANALYSIS OF PLASMID DNA ISOLATED FROM STRAINS OF STAPHYLOCOCCUS AUREUS USING ARTIFICIAL NUCLEASES

Popis výsledku anglicky

Hydrolysis of nucleic acids mediated by artificial nucleases is very important goal in molecular biology, biotechnology and medicine. Some metal and lanthanide ions and their soluble or insoluble complexes (immobilized on solid particles) are attractiveas catalysts for DNA cleavage. The aim of this work was to use of neodymium ions for cleavage of plasmid DNAs. Plasmid DNAs originated from strains of Staphylococcus aureus isolated from clinical and food samples were treated by neodymium ions (Nd3+). Plasmid pUC19 was used as model molecule and linearized pUC19/EcoRI was used as the control of the presence of linear form. Cleavage of plasmid DNA was carried out at different temperatures in HEPES (0.05M) buffer. The reaction time was up to 4 hours. Thereaction was stopped with 0.5 M EDTA (pH 8.0). Plasmid DNA cleavage efficiency was evaluated by convert the supercoiled circular (ccc) form of plasmid DNA into nicked circular form (oc) and linear (lin) one and was monitored on the gel ac

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů