High pressure modification of the simple automated liquid chromatographic system for splitless nano column gradient separations

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F13%3APU106482" target="_blank" >RIV/00216305:26310/13:PU106482 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

High pressure modification of the simple automated liquid chromatographic system for splitless nano column gradient separations

Popis výsledku v původním jazyce

In this work we have modified our concept of simple splitless gradient liquid chromatographic system for micro and nano columns with backpressure up to 50 bar. Presented configuration allows us to run analysis under the pressure up to 400 bar. Experimental configuration consists of an OEM programmable syringe pump equipped with a 250-ul high-pressure syringe and ten-port selector valve. High pressure syringe is connected to central port of selector valve via 0.5 meter long PEEK capillary of 380 um i. d.fixed in horizontal plane. PEEK capillary here serves as gradient generator as well as gradient loop and as sample loop. Mobile phase gradients are created by successive sucking of defined volumes of four water-acetonitrile solutions with gradually changing acetonitrile concentration into the PEEK capillary. Sharp mobile phase boundaries fade out while passing through the valve body and due to the parabolic profile of laminar flow as well as due to diffusion. Available linear gradients

Název v anglickém jazyce

High pressure modification of the simple automated liquid chromatographic system for splitless nano column gradient separations

Popis výsledku anglicky

In this work we have modified our concept of simple splitless gradient liquid chromatographic system for micro and nano columns with backpressure up to 50 bar. Presented configuration allows us to run analysis under the pressure up to 400 bar. Experimental configuration consists of an OEM programmable syringe pump equipped with a 250-ul high-pressure syringe and ten-port selector valve. High pressure syringe is connected to central port of selector valve via 0.5 meter long PEEK capillary of 380 um i. d.fixed in horizontal plane. PEEK capillary here serves as gradient generator as well as gradient loop and as sample loop. Mobile phase gradients are created by successive sucking of defined volumes of four water-acetonitrile solutions with gradually changing acetonitrile concentration into the PEEK capillary. Sharp mobile phase boundaries fade out while passing through the valve body and due to the parabolic profile of laminar flow as well as due to diffusion. Available linear gradients

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemické listy

ISSN

1213-7103

e-ISSN

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Svazek periodika

107

Číslo periodika v rámci svazku

s3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

3

Strana od-do

438-440

Kód UT WoS článku

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EID výsledku v databázi Scopus

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