Development of Simple Splitless Gradient Liquid Chromatographic system

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F12%3APU102164" target="_blank" >RIV/00216305:26310/12:PU102164 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Development of Simple Splitless Gradient Liquid Chromatographic system

Popis výsledku v původním jazyce

A simple gradient liquid chromatographic system for micro and nano column separations has been assembled and tested. The device involves simple splitless gradient generation and sample delivery. Experimental configuration consists of an OEM programmablesyringe pump equipped with a glass microsyringe and ten-port selector valve. Automated system was controlled via LabVIEW development environment. Continuous gradients of mobile phase were created in the syringe barrel by successive sucking of adequate volumes of four mobile phases with gradually decreasing acetonitrile concentration and due to turbulent mixing occurred at the syringe needle-barrel boundary. System performance was evaluated using test mixture of six alkylphenones. Good values of relativestandard deviation of retention times were received (intra-day RSD < 0.3 %, inter-day RSD < 1.4 %). Repeatability of sample injection in the range 0.1 ? 3 ul was evaluated. The peak areas were measured and statistically evaluated (RSD <

Název v anglickém jazyce

Development of Simple Splitless Gradient Liquid Chromatographic system

Popis výsledku anglicky

A simple gradient liquid chromatographic system for micro and nano column separations has been assembled and tested. The device involves simple splitless gradient generation and sample delivery. Experimental configuration consists of an OEM programmablesyringe pump equipped with a glass microsyringe and ten-port selector valve. Automated system was controlled via LabVIEW development environment. Continuous gradients of mobile phase were created in the syringe barrel by successive sucking of adequate volumes of four mobile phases with gradually decreasing acetonitrile concentration and due to turbulent mixing occurred at the syringe needle-barrel boundary. System performance was evaluated using test mixture of six alkylphenones. Good values of relativestandard deviation of retention times were received (intra-day RSD < 0.3 %, inter-day RSD < 1.4 %). Repeatability of sample injection in the range 0.1 ? 3 ul was evaluated. The peak areas were measured and statistically evaluated (RSD <

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Studentská odborná konference Chemie je život - Sborník příspěvků

ISBN

978-80-214-4644-1

ISSN

—

e-ISSN

—

Počet stran výsledku

4

Strana od-do

446-449

Název nakladatele

Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno

Místo vydání

Neuveden

Místo konání akce

Brno

Datum konání akce

7. 12. 2012

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

—