Latest improvements in development of simple splitless gradient liquid chromatographic system for micro and nano columns

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F13%3APU104054" target="_blank" >RIV/00216305:26310/13:PU104054 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Latest improvements in development of simple splitless gradient liquid chromatographic system for micro and nano columns

Popis výsledku v původním jazyce

Our simple liquid chromatographic system for splitless gradient nanocolumn separations at backpressure up to 50 bars was modified to run analysis under the pressure up to 300 bars. Programmable syringe pump was equipped with a 250-ul high-pressure syringe and mobile phase gradients were created and stored in relatively long capillary. Available linear gradients of volumes from 5 to 50 ul traced by uracil are presented. Sample introduction was performed as separate step and real large volume of sample (tens of microliters) could be injected and pre-concentrated on-column. Relative standard deviation of retention times and peak areas were received (RSD < 0.3 % and RSD < 5 % respectively).

Název v anglickém jazyce

Latest improvements in development of simple splitless gradient liquid chromatographic system for micro and nano columns

Popis výsledku anglicky

Our simple liquid chromatographic system for splitless gradient nanocolumn separations at backpressure up to 50 bars was modified to run analysis under the pressure up to 300 bars. Programmable syringe pump was equipped with a 250-ul high-pressure syringe and mobile phase gradients were created and stored in relatively long capillary. Available linear gradients of volumes from 5 to 50 ul traced by uracil are presented. Sample introduction was performed as separate step and real large volume of sample (tens of microliters) could be injected and pre-concentrated on-column. Relative standard deviation of retention times and peak areas were received (RSD < 0.3 % and RSD < 5 % respectively).

Druh

O - Ostatní výsledky

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů