Determination of the reference genes in Streptococcus equi subsp. zooepidemicus for real-time PCR

Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU114977" target="_blank" >RIV/00216305:26310/15:PU114977 - isvavai.cz</a>
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RIV/00216305:26310/15:PU117615
Výsledek na webu
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DOI - Digital Object Identifier
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Jazyk výsledku
angličtina
Název v původním jazyce
Determination of the reference genes in Streptococcus equi subsp. zooepidemicus for real-time PCR
Popis výsledku v původním jazyce
The whole genome expression analysis of Streptococcus equi subsp. zooepidemicus strain ATCC 35246 was performed using DNA microarrays. According to the stability of gene expression 10 genes including gyrA and gyrB were chosen as potential reference genes. Further analysis of expression stability of selected genes was done by real-time PCR. Ct values from PCR analysis were processed in Microsoft Excel and our conclusions were additionally confirmed by GeNorm normalization. Reference gene was chosen
Název v anglickém jazyce
Determination of the reference genes in Streptococcus equi subsp. zooepidemicus for real-time PCR
Popis výsledku anglicky
The whole genome expression analysis of Streptococcus equi subsp. zooepidemicus strain ATCC 35246 was performed using DNA microarrays. According to the stability of gene expression 10 genes including gyrA and gyrB were chosen as potential reference genes. Further analysis of expression stability of selected genes was done by real-time PCR. Ct values from PCR analysis were processed in Microsoft Excel and our conclusions were additionally confirmed by GeNorm normalization. Reference gene was chosen

Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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