SIMPLY METHOD OF DNA ISOLATION FROM LACTIC ACID BACTERIA
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU115275" target="_blank" >RIV/00216305:26310/15:PU115275 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
SIMPLY METHOD OF DNA ISOLATION FROM LACTIC ACID BACTERIA
Popis výsledku v původním jazyce
Identification of bacterial DNA consists of several steps: cell lysis, extraction and purification of DNA followed by the identification of the bacterial species using polymerase chain reaction (PCR). Genomic DNA was isolated from Lactobacillus plantarumCCM 7039, Lactobacillus fermentum CCM 7192, Lactobacillus rhamnosus CCM 1825, Lactobacillus casei subsp. casei CCM 7088, and Bifidobacterium bifidum CCM 3762 by fenol extraction procedure and by the salting out with NaCl solution. Crude cell lysates were prepared using standard procedure and using of laundry powder. The quality of isolated DNA was verified by UV spectrophotometry and by agarose gel electrophoresis. Isolated DNA was used as a template for PCR
Název v anglickém jazyce
SIMPLY METHOD OF DNA ISOLATION FROM LACTIC ACID BACTERIA
Popis výsledku anglicky
Identification of bacterial DNA consists of several steps: cell lysis, extraction and purification of DNA followed by the identification of the bacterial species using polymerase chain reaction (PCR). Genomic DNA was isolated from Lactobacillus plantarumCCM 7039, Lactobacillus fermentum CCM 7192, Lactobacillus rhamnosus CCM 1825, Lactobacillus casei subsp. casei CCM 7088, and Bifidobacterium bifidum CCM 3762 by fenol extraction procedure and by the salting out with NaCl solution. Crude cell lysates were prepared using standard procedure and using of laundry powder. The quality of isolated DNA was verified by UV spectrophotometry and by agarose gel electrophoresis. Isolated DNA was used as a template for PCR
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů