Detection of Lactobacillus acidophilus species in the probiotic meat product

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU115346" target="_blank" >RIV/00216305:26310/15:PU115346 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26310/15:PU122752

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection of Lactobacillus acidophilus species in the probiotic meat product

Popis výsledku v původním jazyce

Probiotic bacteria have beneficial effects on human health. They positively influence the host immune system and the composition of intestinal microflora. They can reach the gastrointestinal tract with food. Probiotic bacteria are found in dairy productsand food supplements most often. They can be used in other matrices including meat products. The aim of this study was to detect Lactobacillus acidophilus cells in probiotic fermented meat product ?Ovčácká klobása? using the PCR method. Attention was paid to the preparation of the sample (homogenization, lysis of cells) and optimization of methods for the isolation of DNA in quality suitable for PCR using magnetic microparticles. The use of magnetic particles with immobilized antibody antiLactobacilluswas also tested for Lactobacillius acidophilus bacterial cells isolation from homogenized product. Bacterial cells bound on the magnetic particles were identified using PCR. Specific PCR products for the species Lactobacillus acidophilus

Název v anglickém jazyce

Detection of Lactobacillus acidophilus species in the probiotic meat product

Popis výsledku anglicky

Probiotic bacteria have beneficial effects on human health. They positively influence the host immune system and the composition of intestinal microflora. They can reach the gastrointestinal tract with food. Probiotic bacteria are found in dairy productsand food supplements most often. They can be used in other matrices including meat products. The aim of this study was to detect Lactobacillus acidophilus cells in probiotic fermented meat product ?Ovčácká klobása? using the PCR method. Attention was paid to the preparation of the sample (homogenization, lysis of cells) and optimization of methods for the isolation of DNA in quality suitable for PCR using magnetic microparticles. The use of magnetic particles with immobilized antibody antiLactobacilluswas also tested for Lactobacillius acidophilus bacterial cells isolation from homogenized product. Bacterial cells bound on the magnetic particles were identified using PCR. Specific PCR products for the species Lactobacillus acidophilus

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GP13-32840P" target="_blank" >GP13-32840P: Vývoj metody pro studium enkapsulace magnetických nano- a mikročástic pomocí polymerázové řetězové reakce v reálném čase</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů