The evaluation of magnetic particles suitable for DNA isolation using real time PCR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU115351" target="_blank" >RIV/00216305:26310/15:PU115351 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26310/15:PU122753

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The evaluation of magnetic particles suitable for DNA isolation using real time PCR

Popis výsledku v původním jazyce

The problem of pure DNA preparation from complex matrix and the effects of the inhibitors from complex biological samples can be reduced by the selection of the appropriate nucleic acid isolation method. Numerous solid phase systems, especially magneticparticles, have been used to a great extent for DNA isolation. Magnetically driven separation techniques using micro- and nano- sized magnetic solid carriers have become one of modern methods to speed up and facilitate the previously used separation andpurification procedures. Magnetic particles are mostly based on magnetic iron oxide (magnetic core) covered by polymer surfaces suitable for modification by different functional groups (e.g. ?COOH, -NH2). Free or incomplete encapsulated cores and some components applied in the preparation of magnetic particles can interfere with polymerase chain reaction (PCR). Magnetic particles with complete covering of magnetic nuclei do not interfere in PCR, and thus such particles are preferred.

Název v anglickém jazyce

The evaluation of magnetic particles suitable for DNA isolation using real time PCR

Popis výsledku anglicky

The problem of pure DNA preparation from complex matrix and the effects of the inhibitors from complex biological samples can be reduced by the selection of the appropriate nucleic acid isolation method. Numerous solid phase systems, especially magneticparticles, have been used to a great extent for DNA isolation. Magnetically driven separation techniques using micro- and nano- sized magnetic solid carriers have become one of modern methods to speed up and facilitate the previously used separation andpurification procedures. Magnetic particles are mostly based on magnetic iron oxide (magnetic core) covered by polymer surfaces suitable for modification by different functional groups (e.g. ?COOH, -NH2). Free or incomplete encapsulated cores and some components applied in the preparation of magnetic particles can interfere with polymerase chain reaction (PCR). Magnetic particles with complete covering of magnetic nuclei do not interfere in PCR, and thus such particles are preferred.

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GP13-32840P" target="_blank" >GP13-32840P: Vývoj metody pro studium enkapsulace magnetických nano- a mikročástic pomocí polymerázové řetězové reakce v reálném čase</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů