Study on the encapsulation of magnetic cores using real time polymerase chain reaction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F14%3APU112201" target="_blank" >RIV/00216305:26310/14:PU112201 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Study on the encapsulation of magnetic cores using real time polymerase chain reaction

Popis výsledku v původním jazyce

Solid phase magnetic driven separation techniques in combination with molecular diagnostic methods (polymerase chain reaction, PCR) have become popular to speed up and facilitate the nucleic acids separation and purification procedures. Magnetic particles are mostly based on magnetic iron oxide (magnetic core) covered by polymer surfaces suitable for modification by different functional groups (e.g. ?COOH, -NH2). Free or incomplete encapsulated cores and some components applied in the preparation of magnetic particles can interfere with polymerase chain reaction [1, 2]. Therefore perfectly encapsulation of magnetic cores and removing all potential PCR inhibiting components are key factors for the application of magnetic particles in molecular diagnostics. For this reasons, polymerase chain reaction in quantitative real time (qPCR) and the software supplied with the qPCR, were used for the estimation of the inhibitory effect of different types of magnetic microparticles. The set of magn

Název v anglickém jazyce

Study on the encapsulation of magnetic cores using real time polymerase chain reaction

Popis výsledku anglicky

Solid phase magnetic driven separation techniques in combination with molecular diagnostic methods (polymerase chain reaction, PCR) have become popular to speed up and facilitate the nucleic acids separation and purification procedures. Magnetic particles are mostly based on magnetic iron oxide (magnetic core) covered by polymer surfaces suitable for modification by different functional groups (e.g. ?COOH, -NH2). Free or incomplete encapsulated cores and some components applied in the preparation of magnetic particles can interfere with polymerase chain reaction [1, 2]. Therefore perfectly encapsulation of magnetic cores and removing all potential PCR inhibiting components are key factors for the application of magnetic particles in molecular diagnostics. For this reasons, polymerase chain reaction in quantitative real time (qPCR) and the software supplied with the qPCR, were used for the estimation of the inhibitory effect of different types of magnetic microparticles. The set of magn

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GP13-32840P" target="_blank" >GP13-32840P: Vývoj metody pro studium enkapsulace magnetických nano- a mikročástic pomocí polymerázové řetězové reakce v reálném čase</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů