The study of biocompatibility of magnetic particles using amplification methods
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU115587" target="_blank" >RIV/00216305:26310/15:PU115587 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216305:26310/15:PU122751
Výsledek na webu
<a href="http://www.ceitec.eu/a5-abstracts-book-3d/f33102" target="_blank" >http://www.ceitec.eu/a5-abstracts-book-3d/f33102</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The study of biocompatibility of magnetic particles using amplification methods
Popis výsledku v původním jazyce
Magnetic particles for biotechnology applications usually consist of a number of components: magnetic cores are covered with a variety of functionalized low-or high- molecular compounds (polymer layer). Iron oxides and some substances used to stabilize magnetic cores can reduce biocompatibility of the proposed particles; therefore perfectly encapsulated cores are very important for their application. The real-time polymerase chain reaction (qPCR) is very sensitive for the presence a number of chemicals.The increasing inhibitors concentration decreases the amplification efficiency and thus the reaction curve slope decreases. The experiments for the estimation magnetic nano- and microparticles inhibitory effect or biocompatibility respectively on the course of real-time PCR were proposed. The set of magnetic non-porous microspheres covered by different content of carboxyl groups, PEGylated monodisperse magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspher
Název v anglickém jazyce
The study of biocompatibility of magnetic particles using amplification methods
Popis výsledku anglicky
Magnetic particles for biotechnology applications usually consist of a number of components: magnetic cores are covered with a variety of functionalized low-or high- molecular compounds (polymer layer). Iron oxides and some substances used to stabilize magnetic cores can reduce biocompatibility of the proposed particles; therefore perfectly encapsulated cores are very important for their application. The real-time polymerase chain reaction (qPCR) is very sensitive for the presence a number of chemicals.The increasing inhibitors concentration decreases the amplification efficiency and thus the reaction curve slope decreases. The experiments for the estimation magnetic nano- and microparticles inhibitory effect or biocompatibility respectively on the course of real-time PCR were proposed. The set of magnetic non-porous microspheres covered by different content of carboxyl groups, PEGylated monodisperse magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspher
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GP13-32840P" target="_blank" >GP13-32840P: Vývoj metody pro studium enkapsulace magnetických nano- a mikročástic pomocí polymerázové řetězové reakce v reálném čase</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů