The study of encapsulation magnetic nano- and microparticles using real time polymerase chain reaction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F14%3APU111044" target="_blank" >RIV/00216305:26310/14:PU111044 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The study of encapsulation magnetic nano- and microparticles using real time polymerase chain reaction

Popis výsledku v původním jazyce

Nano- and microparticles for biotechnology applications usually consist of a number of components: superparamagnetic core, protective coating and the surface functionality. Magnetic cores are covered with a variety of functionalized low-or high-molecularcompounds (polymer layer). Iron oxides and some substances used to stabilize magnetic cores can reduce biocompatibility of the proposed particles; therefore perfectly encapsulated cores are very important for their application. The aim of work was the development of simple method for the study of magnetic cores encapsulation of nano- and microparticles suitable for applications in molecular diagnostics. The degree of magnetic particles coverage with a variety of low-or high molecular weight compounds (polymer layer) was verified by real time polymerase chain reaction (qPCR). The proposed method is based on estimation of DNA amplification effectiveness in qPCR. Serial dilutions of different types of magnetic particles, such as (hydrophi

Název v anglickém jazyce

The study of encapsulation magnetic nano- and microparticles using real time polymerase chain reaction

Popis výsledku anglicky

Nano- and microparticles for biotechnology applications usually consist of a number of components: superparamagnetic core, protective coating and the surface functionality. Magnetic cores are covered with a variety of functionalized low-or high-molecularcompounds (polymer layer). Iron oxides and some substances used to stabilize magnetic cores can reduce biocompatibility of the proposed particles; therefore perfectly encapsulated cores are very important for their application. The aim of work was the development of simple method for the study of magnetic cores encapsulation of nano- and microparticles suitable for applications in molecular diagnostics. The degree of magnetic particles coverage with a variety of low-or high molecular weight compounds (polymer layer) was verified by real time polymerase chain reaction (qPCR). The proposed method is based on estimation of DNA amplification effectiveness in qPCR. Serial dilutions of different types of magnetic particles, such as (hydrophi

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GP13-32840P" target="_blank" >GP13-32840P: Vývoj metody pro studium enkapsulace magnetických nano- a mikročástic pomocí polymerázové řetězové reakce v reálném čase</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů