Optimalization of HRM-PCR method for identification lactic acid bacteria from complex samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F16%3APU124177" target="_blank" >RIV/00216305:26310/16:PU124177 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Optimalization of HRM-PCR method for identification lactic acid bacteria from complex samples

Popis výsledku v původním jazyce

Molecular based differentiation of various bacterial species is important mainly in diagnostic, epidemiological and food quality control studies, particulary where phenotype makes the identification of bacteria difficult. Molecular methods based on the amplification of 16S ribosomal RNA gene analysis are more rapid, reliable, and reproducible and have greater sensitivity. High resolution melting (HRM) curve analysis is a simple, low-ccost, closed tube method for amplicon discrimination and easy integration with real.time polymerase chain reaction (HRM-PCR). In this contribution we report rapid species identification of lactic acid bacteria using HRM-PCR in complex food samples. Three different DNA isolation methods were used in this work: phenol extraction, separation using magnetic particles and commercial kit. Three sets of primers targeted hypervariable region (V1-F - V1-R, V3-F - V3-R, V6-F - V6-R) were tested for amplification of the 16S rRNA gene. Presented method was optimized on 6 Reference co

Název v anglickém jazyce

Optimalization of HRM-PCR method for identification lactic acid bacteria from complex samples

Popis výsledku anglicky

Molecular based differentiation of various bacterial species is important mainly in diagnostic, epidemiological and food quality control studies, particulary where phenotype makes the identification of bacteria difficult. Molecular methods based on the amplification of 16S ribosomal RNA gene analysis are more rapid, reliable, and reproducible and have greater sensitivity. High resolution melting (HRM) curve analysis is a simple, low-ccost, closed tube method for amplicon discrimination and easy integration with real.time polymerase chain reaction (HRM-PCR). In this contribution we report rapid species identification of lactic acid bacteria using HRM-PCR in complex food samples. Three different DNA isolation methods were used in this work: phenol extraction, separation using magnetic particles and commercial kit. Three sets of primers targeted hypervariable region (V1-F - V1-R, V3-F - V3-R, V6-F - V6-R) were tested for amplification of the 16S rRNA gene. Presented method was optimized on 6 Reference co

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů