High resolution melting analysis for identification of strains beloging to the Lactobacillus casei group

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F18%3APU127970" target="_blank" >RIV/00216305:26310/18:PU127970 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

High resolution melting analysis for identification of strains beloging to the Lactobacillus casei group

Popis výsledku v původním jazyce

Molecular methods based on the amplification of 16S ribosomal RNA gene analysis are more rapid, reliable, and reproducible and have greater sensitivity. High resolution melting (HRM) curve analysis is a simple, low-cost, closed tube method for amplicon discrimination and easy connection with real-time polymerase chain reaction (PCR). In this contribution, we report rapid species identification of strains belonging to the Lactobacillus casei group using HRM-PCR. Three different DNA isolation methods were used in this work: phenol extraction, separation using magnetic particles and commercial kit. Two sets of targeted gene fragments primers (groEL, BSH) were tested for amplification of the 16S rRNA gene. Use of groEL primers pair successfully identify strains belong to the Lactobacillus casei group. The variance between used extraction methods for evidence of HRM curves was found.

Název v anglickém jazyce

High resolution melting analysis for identification of strains beloging to the Lactobacillus casei group

Popis výsledku anglicky

Molecular methods based on the amplification of 16S ribosomal RNA gene analysis are more rapid, reliable, and reproducible and have greater sensitivity. High resolution melting (HRM) curve analysis is a simple, low-cost, closed tube method for amplicon discrimination and easy connection with real-time polymerase chain reaction (PCR). In this contribution, we report rapid species identification of strains belonging to the Lactobacillus casei group using HRM-PCR. Three different DNA isolation methods were used in this work: phenol extraction, separation using magnetic particles and commercial kit. Two sets of targeted gene fragments primers (groEL, BSH) were tested for amplification of the 16S rRNA gene. Use of groEL primers pair successfully identify strains belong to the Lactobacillus casei group. The variance between used extraction methods for evidence of HRM curves was found.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Študentská vedecká konferencia PriF UK 2018 Zborník recenzovaných príspevkov

ISBN

978-80-223-4517-0

ISSN

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e-ISSN

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Počet stran výsledku

1652

Strana od-do

302-307

Název nakladatele

Neuveden

Místo vydání

Neuveden

Místo konání akce

Bratislava

Datum konání akce

25. 4. 2018

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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